การทวนสอบวิธีและการศึกษาปริมาณการปนเปื้อนสารอะฟลาทอกซินเอ็ม1 ในนมและผลิตภัณฑ์นม

Apisthaa Changsuphan

doi:10.60136/bas.v10.2021.143

Authors

Apisthaa Changsuphan Department of Science Service

DOI:

https://doi.org/10.60136/bas.v10.2021.143

Keywords:

Aflatoxin M1, High-performance liquid chromatography, milk and milk product

Abstract

Aflatoxin M₁ (AFM₁) has been identified as toxic substance, which produced from the metabolism of Aflatoxin B1 (AFB₁) in mammals that consumed contaminant feed. AFB₁ is metabolized by enzyme in liver cells and transformed to AFM₁ then excrete through mammal milk. There are both indirect and direct adverse effected to the consumer who consumed meat and milk contaminated with Aflatoxin. Continuously consumtion can be resulted in accumulation in body hence contribute to liver cancer. The Codex Alimentarius Commission has established standard of AFM₁in milk product to less than 0.5 microgram/Kilogram.

The aim of this study was to investigate the optimum condition and verification for AFM₁ testing method which using HPLC. The study showed the linear correlation between the concentrations from 0.1 to 50 microgram/Liter, limit of detection (LOD) of 0.001 microgram/Liter and limit of quantitation (LOQ) of 0.004 microgram/Liter. The study also demonstrated acceptable percent recovery, precision by relative standard deviation (%RSD). The uncertainty of testing also included in this study. Animal feed samples were taken farms dairy farm in Nakorn-Rachasima and Saraburi provinces. While milk and milk products were sampled from markets in Bangkok, Nakorn-Rachasima and Saraburi provinces Results illustrated the AFM₁ concentrations range from not detected to 0.0552 microgram/Kilogram.

References

Agents Classified by the IARC Monographs, Vol. 1–125, p. 15/39

American Scientific Research Journal for Engineering, Technology and Sciences (ASRJETS) (2013) Vol. 7, No. 1, p. 1-32

Badea, M., Micheli, L., Messia, M.C., Candigliota, T., Marconi, E., Mottram, T., Velasco-Garcia, M., Moscone, D., Palleschi, G., 2004. Aflatoxin M1 determination in raw milk using a flow-injection immunoassay system Analytica Chimica Acta, 520, p.141–148

Commission Regulation (EC) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs (Text with EEA relevance) (OJ L 364, 20.12.2006, p. 5)

Dragacci, S. and Fremy, J. M., 1996. Application of Immunoaffinity Column Cleanup to Aflatoxin M1 Determination and Survey in Cheese Journal of Food Protection, Vol. 59, No.9, p. 1011-1013

Dragacci, S., Grosso, F. and Gilbert, J., 2001. Immunoaffinity Column Cleanup with Liquid Chromatography for Determination of AflatoxinM1 in Liquid Milk: Collaborative Study Journal of AOAC International Vol. 84, No. 2, p. 437- 443

Food and Agriculture Organization and World Health Organization. Codex Alimentarius Commission. Agenda Item 5, CX/CF 10/4/5: FAO/WHO, March 2010, p.1-25.

General Standard for Contaminants and Toxins in Food and Feed Codex Alimentarius International Food Standards. CXS 193-1995 Amended in 2019, p.32

Hamid Mohammadi, 2011. A Review of Aflatoxin M1, Milk, and Milk Products Aflatoxins - Biochemistry and Molecular Biology, No. 19, p.397-414

Iha, M.H., Barbosa,C.B. and Favaro, R.M.D., Mary W. , 2011. Trucksess Chromatographic Method for the Determination of Aflatoxin M1 in Cheese, Yogurt, and Dairy Beverages Journal of AOAC International Vol. 94, No. 5

Iqbal, S.Z., Asi, M.R., 2013.Assessment of aflatoxin M1 in milk and milk products from Punjab, Pakistan Food Control, Vol.30, p.235-239

Muscarella, M., Magroa, S.L., Palermo, C., Centonze, D., 2007. Validation according to European Commission Decision 2002/657/EC of a confirmatory method for aflatoxin M1 in milk based on immunoaffinity columns and high-performance liquid chromatography with fluorescence detection Analytica Chimica Acta, 594, p.257–264

Nemati, M., Mehran, M.A., Hamed, P.K., Masoud, A., 2010. A survey on the occurrence of aflatoxin M1 in milk samples in Ardabil, Iran Food control, Vol. 21, p.1022-1024.