การปรับสภาพเปลือกข้าวโพดและเปลือกมะพร้าวด้วยของเหลวไอออนิก เพื่อเพิ่มผลผลิตน้ำตาลกลูโคส

Watcharee Katinonkul; Jenjira Phuriragpitikhon

doi:10.60136/bas.v2.2013.267

Authors

Watcharee Katinonkul Department of Science Service
Jenjira Phuriragpitikhon Department of Science Service

DOI:

https://doi.org/10.60136/bas.v2.2013.267

Keywords:

Lignocellulosic materials, Pretreatment, Ionic liquid, Enzymatic hydrolysis

Abstract

The study demonstrates the ionic liquid pretreatment for lignocellulosic materials like corn husk and coconut husk in order to optimize the yield of glucose recovery. Two ionic liquids: 1-butyl-3-methylimidazolium chloride (BmimCl) and 1-ethyl-3-methylimidazolium acetate (EmimOAC) were studied. The pretreated materials were enzymatically hydrolyzed using Celluclast 1.5L and Novozymes 188. It was found that the optimum pretreatment condition for corn husk and coconut husk were accomplished using EmimoAc at the temperature of 130°C and duration of 2 hours, resulted in the maximum glucose recovery at 68.2 and 62.8%, respectively. In addition, using ionic liquid pretreatment could decrease the lignin content in corn husk and coconut husk structures for 9.2 and 14.6%, respectively.

References

Dadi, A.P., Varanasi, S. and Schall, C.A. "Enhancement of cellulose saccharification kinetics using an ionic liquid pretreatment step." Biotechnol.Bioeng. 2006, 95, 904-10.

Mosier, N., Wyman, C., Dale, B., Elander, R., Lee, Y.Y., Holtzapple, M. and Ladisch, M. "Features of promising technologies for pretreatment of lignocellulosic biomass." Bioresour. Technol. 2005, 96, 673-86.

Swatloski, R.P., Spear, S.K., Holbrey, J.D. and Rogers, R.D. "Dissolution of cellulose with ionic liquids.” J. Am.Chem.Soc., 2002, 124, 4974-75.

NREL, Chemical analysis and testing laboratory analytical procedure (CAT). Golden, CO, USA: National Renewable Energy Laboratory; 2004.

Zhu., L., OʻDwyer, J.P., Chang, V.S., Granda, C.B. and Holtzapple, M.T. "Structural features affecting biomass enzymatic digestibility." Bioresour. Technol., 2008, 99, 3817-28.

Zhao, H., Jones, C.L., Baker, G.A., Xia, S., Olubajo, O. and Person, V.N. "Regenerating cellulose from ionic liquids for an accelerated enzymatic hydrolysis." J. Biotechnol., 2009, 139, 47-54.

Narenda, R. and Yiqi, Y. “Method of making natural cellulosic fiber bundles from cellulosic source." United State Patent, 2001, US 7887672 B2

Pilanee, V., Waraporn, A., Nanthaya, C., Wuttinunt, K. and Sarima, S. "The potential of coconut husk utilization for bioethanol production." Kasetsart J. (Ngt. Sci.), 2011, 45,159-64.