Method validation for determination of sucralose in beverage by high performance liquid chromatography

Abstract

The determination of sucralose in beverages was performed and validated by the modified standard method based on BS EN 16155:2012 Foodstuffs - Determination of sucralose - High performance liquid chromatographic method. The linear calibration curve was obtained in concentrations ranging from 0 to 400 mg/L. The limit of detection (LOD) and the limit of quantitation (LOQ) were 4.0 and 15.0 mg/kg, respectively. The precision and accuracy studies were performed by spiking 3 concentration levels in the samples (25, 250, and 2,000 mg/kg). The recoveries were obtained from 92.0 to 101.6%, 98.8 to 99.4% and 98.0 to 99.8%, respectively. The precision calculated as a relative standard deviation of repeatability (%RSD_r) found good repeatability at 3.799%, 0.169%, and 0.586%. The HORRAT values were 0.824, 0.052 and 0.246, respectively. The percent recovery, precision, and HORRAT value were acceptable and complied with the requirement of EURACHEM/CITAC Guide: 2012. The percentage of expanded uncertainty was 3.7% at a 95% confidence interval (k=2). Moreover, thirty samples from local markets were analyzed following this validated method and found that sucralose concentration ranged from not detected or less than 4.0 to 172.7 mg/kg. The result of this study showed the modified methods were fit for the intended use.