การตรวจหาปริมาณสารไพมารีอะโรมาติกเอมีนใน วัสดุสัมผัสอาหารด้วยเทคนิคอัลตราไฮเพอร์ฟอร์แมนซ์ลิควิด โครมาโทกราฟี-แทนเดมแมสสเปกโทรเมตรี

Jutathip Lapviboonsuk; Patnipa Wongpichai; Sompop Lapviboonsuk

doi:10.60136/bas.v13.2024.1129

Authors

Jutathip Lapviboonsuk Division of Food Products and Food Contact Material, Department of Science Service
Patnipa Wongpichai Division of Food Products and Food Contact Material, Department of Science Service
Sompop Lapviboonsuk Division of Food Products and Food Contact Material, Department of Science Service

DOI:

https://doi.org/10.60136/bas.v13.2024.1129

Keywords:

Primary aromatic amine, Food contact materials, Polyamide, Black nylon, Liquid chromatography-tandem mass spectrometry

Abstract

The determination of primary aromatic amines (PAAs) in food contact materials involved the examination of ten specific types of PAAS: Aniline (ANL), o-Anisidine (o-ASD), 4-chloro-aniline (4-CA), 4-chloro-o-toluidine (4-COT), 2,6-dimethylaniline (2,6-DMA), 4,4’-methylenedianiline (4,4’-MDA), 4,4-methylenedi-o-toluidine (4,4’- MDOT), 2-methoxy-5-methylaniline (2-M-5-MA), m-phenylenediamine (m-PDA) and o-toluidine (o-T). The extraction solution for PAAs, known as food simulant, was 3% (w/v) acetic acid at 100 °C, which analyzed by ultra-high performance liquid chromatography-tandem mass spectrometry. The method validation according to Eurachem Guide: The Fitness for Purpose of Analytical Methods, 2014, limit of detection and limit of quantitation were 2 and 10 microgram per kilogram, respectively. To prove linearity within the range of 2.00 - 20.0 nanograms per milliliter, each type of PAA was assessed, yielding a coefficient of determination above 0.995. Moreover, accuracy and precision were investigated at 3 concentration levels: 10, 25 and 50 micrograms per kilogram which met the requirement of 2002/657/EC. Therefore, this test method fit in the application of PAAS testing.

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