METHODS FOR THE ANALYSIS OF COLLAGEN IN FORTIFIED BEVERAGES WITH COLLAGEN HYDROLYSATED USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTOR

Authors

  • JANYA SANGKHIAW Food Product Research and Development Institute Kasetsart University

Keywords:

Collagen hydrolysated, High-Performance Liquid Chromatography, Method validation

Abstract

This research aimed to study methods for determination of the collagen content of beverage products containing hydrolyzed collagen. The use of FMOC-Cl derivatives and high performance liquid chromatography was analyzed with a fluorescence detector and multiplied by a conversion factor of 7.25 to obtain the amount of collagen in the sample. It was found that beverage products commercially available had collagen content in the range from 0.1-25.0%. A method was validated for the collagen content had the calibration curve for 4-Hydroxy-L-proline was found to be linear in the concentrations ranging from 0.5-50 mg/L, with a correlation coefficient (R2) of 0.996. The value of LOD and LOQ were 0.004% and 0.013%, respectively. The repeatability (use as a precision) of the method was observed and the standard deviation of concentration (%RSD) for Intra-day and Inter-day was 8.16% and 7.48%, respectively. The accuracy determined from the percentage of return ranged from 80-110% at the level of the substance of interest not more than 10 mg/L and the Horwitz ratio of less than 2. It was shown that the method for determining of the collagen content in beverage products containing collagen hydrolyzes was accurate and they are appropriate to be used in routine operations and comply with laboratory standards.

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Published

2021-03-31

How to Cite

[1]
J. SANGKHIAW, “METHODS FOR THE ANALYSIS OF COLLAGEN IN FORTIFIED BEVERAGES WITH COLLAGEN HYDROLYSATED USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTOR”, JSciTech, vol. 5, no. 1, pp. 34–45, Mar. 2021.

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Section

Research Articles