การทดสอบสารพฤกษเคมี ปริมาณฟลาโวนอยด์และสารประกอบฟีนอลรวม และฤทธิ์ยับยั้งกิจกรรม ของเอนไซม์แอลฟาอะไมเลสและแอลฟากลูโคซิเดสของสารสกัดจากใบกระโดน

Rung-arun Treepop; Supannee   Aoki; Parinya   Moonsin; Phukphon   Munglue

Authors

Rung-arun Treepop Program of Science Education, Faculty of Science, Ubon Ratchathani Rajabhat University
Supannee Aoki Program of Biology, Faculty of Science, Ubon Ratchathani Rajabhat University
Parinya Moonsin Program of Biology, Faculty of Science, Ubon Ratchathani Rajabhat University
Phukphon Munglue Program of Biology, Faculty of Science, Ubon Ratchathani Rajabhat University

Keywords:

Phytochemicals, Tummy-Wood, alpha-Amylase, alpha-Glucosidase

Abstract

This study aimed to evaluate phytochemicals, total flavonoid and phenolic compound contents, and alpha-amylase and alpha-glucosidase inhibitory activities of Tummy-Wood (Careya sphaerica Roxb.) leaf extract (TLE). Leaf samples were methanolic extracted. Phytochemical screening presents glycosides, alkaloids, flavonoids, phenolic compounds, saponins, terpenoids, and coumarins. Total phenolic and flavonoid contents of TLE were 2.88 ± 0.59 mg GAE/g of extract and 10.02 ± 0.58 mg QE/g of extract, respectively. The tannin content detected in the extract was 1.29 ± 0.14 mg/100 mg of dry plant weight. The IC₅₀ values of alpha-amylase and alpha-glucosidase inhibitory activities produced by TLE were 0.42 ± 0.01 and 2.97 ± 0.09 mg/ml, respectively. These results indicated that TLE might be an excellent source of various phytochemicals with antihyperglycemic potential.

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