Application of MALDI-TOF MS to Rapid Identification of Streptomyces Application of MALDI-TOF MS to Rapid Identification of Streptomyces
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Abstract
This study aimed to describe the use of MALDI-TOF MS for the rapid identification of Streptomyces isolates from the durian orchard in Chanthaburi province, Thailand. Twelve Streptomyces were taxonomically studied based on their phenotypic characteristics. Firstly colonies are relatively smooth surfaced but later they develop a weft of aerial mycelium that may appear floccose, powdery, or velvety. The results shown that 12 isolates were rapidly identified (<15 minutes) with high scores between 1.72 to 2.56. Twelve isolates were identified as S. matensis, S. albogriseolus, S. matensis, S. cavourensis, S, albidoflavus, S. albidoflavus, S. cavourensis, S. cavourensis, S. cavourensis, S. viridodiastaticus, S. griseoincarnatus, S. cavourensis (Score value are 1.72, 1.79, 2.28, 2.23, 2.13, 2.28, 2.60, 2.06, 2.17, 1.77, 1.75 and 2.56 respectively). These results indicate that MALDI-TOF MS could be used as a cost–effective tool for rapid identification of Streptomyces isolates.
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