Method validation for determination of phenol in plastic products using high performance liquid chromatography

Abstract

The method validation for determination of phenol in plastic products was performed by the modified method based on European Standard EN 71-11, 2005: Safety of toys Method using high-performance liquid chromatography. From the study, the calibration graph showed a good linear range from 0.2 to 10 mg/L. The limit of detection was 0.03 mg/L and limit of quantitation was 0.2 mg/L. Bias and precision study were performed by using spike samples at 3 concentration levels (0.2, 4.0 and 10.0 mg/L). The recoveries of phenol in plastic were between 80-120%. Relative standard deviations were between 0.01-0.04. The percentage recovery and relative standard deviation were within acceptance limit. The result showed that there is no matrix effect. Then data from bias and precision study could be used to estimate measurement uncertainty of the method. The expanded uncertainty of phenol at the confidence level of 95% was less than 20% which corresponding to the target uncertainty. Therefore, this method was fit for the intended use.