Total Phenolic and Flavonoid Contents, Antioxidant Activity and Alpha-Amylase Inhibitory Activity of Four Local Rice Varieties

Authors

  • Porntip Khampa 1 Program of Science Education, Faculty of Science, Ubon Ratchathani Rajabhat University
  • Aroon Jankham Program of Chemistry, Faculty of Science, Ubon Ratchathani Rajabhat University
  • Supannee Aoki Program of Biology, Faculty of Science, Ubon Ratchathani Rajabhat University
  • Phukphon Munglue Program of Biology, Faculty of Science, Ubon Ratchathani Rajabhat University

Keywords:

local rice varieties, phytochemicals, antioxidation, alpha-amylase

Abstract

This study examined total phenolic and flavonoid contents, antioxidant activity, and alpha-amylase inhibitory activity of four local rice varieties, including Selabporn, Kusuma, Phetratri, and Vessantara. Rice samples were extracted with distilled water. The highest contents of total phenolic compound and total flavonoid were found in Selabporn (20.72±0.31 mg GAE/20 g gain weight and 72.62±8.19 mg QE/20 g gain weight, respectively) when compared with other rice varieties. Additionally, antioxidant activity tested by using DPPH assay and alpha-amylase inhibitory activity of Selabporn showed the lowest IC50 values of 139.24±1.53 µg/mL and 4.78±0.44 mg/mL, respectively. The results of this study indicated the antioxidant property and alpha-amylase inhibitory activities of four local rice varieties, which may have a potential functional food for maintaining the blood glucose level.

References

ฉวีวรรณ วุฒิญาโณ. (2543). ข้าวพื้นเมืองไทย: เอกสารวิชาการ. กรุงเทพฯ: กรมวิชาการเกษตร สถาบันวิจัยข้าว ศูนย์วิจัยข้าวปทุมธานี ศูนย์ปฏิบัติการและเก็บเมล็ดเชื้อพันธุ์ข้าวแห่งชาติ.

ประพฤติ พรหมสมบูรณ์, ทรงศักดิ์ จันทร์อุดม, อนุสสรณ์ วิเศษสิงห์, สุธัญญา พรหมสมบูรณ์ และคัชชา กาญจนจันทร์. (2559). การรวบรวมพันธุ์และศึกษาลักษณะทางการเกษตรของข้าว (Oryza sativa L.) พันธุ์พื้นเมืองไทย. วารสารเกษตรพระจอมเกล้า, 34(3), 126-132. Retrieved from https://li01.tci-thaijo.org/index.php/agritechjournal/article/view/181006

สุไฮนีย์ เบญจเหม, เทวี ทองแดง คาร์ริลา และเนตรนภิส อ๋องสุวรรณ. (2557). ผลของวิธีการให้ความร้อนและระยะเวลาต่อคุณภาพของน้ำสกัดจากข้าวเหนียวดำ [เอกสารนำเสนอ]. การประชุมทางวิชาการของมหาวิทยาลัยเกษตรศาสตร์ ครั้งที่ 52, กรุงเทพฯ, ประเทศไทย. Retrieved from https://kukrdb.lib.ku.ac.th/proceedings/KUCON/search_detail/ dowload_digital_file/13668/89411

Awan, T.H., Ahmadizadeh, M., Jabran, K., Hashim, S., & Chauhan, B.S. (2017). Domestication and development of rice varieties. In: Chauhan, B., Jabran, K., Mahajan, G. (eds). Rice Production Worldwide. Springer, Cham. https://doi.org/10.1007/978-3-319-47516-5_9

Bhat, F.M., & Riar, C.S. (2017). Characterizing the pigmented traditional rice varieties grown in temperate regions of Kashmir (India) for free and bound phenolics compounds and in vitro antioxidant properties. Journal of cereal science, 76, 253-262. https://doi.org/10.1016/j.jcs.2017.06.018

Budhwar, S., Chakraborty, M., Sethi, K., & Chatterjee, A. (2020). Antidiabetic properties of rice and wheat bran-A review. Journal of food biochemistry, 44(10), e13424. https://doi.org/10.1111/jfbc.13424

Butsat, S., & Siriamornpun, S. (2010). Phenolic acids and antioxidant activities in husk of different Thai rice varieties. Food science and technology international, 16(4), 329-336. https://doi.org/10.1177/1082013210366966

Deng, G.F., Xu, X. R., Zhang, Y., Li, D., Gan, R.Y., & Li, H.B. (2013). Phenolic compounds and bioactivities of pigmented rice. Critical reviews in food science and nutrition, 53(3), 296-306. https://doi.org/10.1080/10408398.2010. 529624

Hao, J., Zhu, H., Zhang, Z., Yang, S., & Li, H. (2015). Identification of anthocyanins in black rice (Oryza sativa L.) by UPLC/Q-TOF-MS and their in vitro and in vivo antioxidant activities. Journal of cereal science, 64, 92-99. https://doi.org/10.1016/j.jcs.2015.05.003

Khantham, C., Linsaenkart, P., Chaitep ,T.,…, & Ruksiriwanich, W. (2022). Antioxidation, anti-inflammation, and regulation of SRD5A gene expression of Oryza sativa cv. bue Bang 3 CMU husk and bran extracts as androgenetic alopecia molecular treatment substances. Plants, 11(3), 330. https://doi.org/10.3390/ plants11030330

Liu, M., Hu, B., Zhang, H., Zhang, Y., Wang, L., Qian, H., & Qi, X. (2017). Inhibition study of red rice polyphenols on pancreatic α-amylase activity by kinetic analysis and molecular docking. Journal of cereal science, 76, 186-192. https://doi.org/10.1016/j.jcs.2017.04.011

Mackon, E., Jeazet Dongho Epse Mackon, G.C., Ma, Y., …, & Liu, P. (2021). Recent insights into anthocyanin pigmentation, synthesis, trafficking, and regulatory mechanisms in rice (Oryza sativa L.) caryopsis. Biomolecules, 11(3), 394. https://doi.org/10.3390/biom11030394

Munglue, P., Rattana, K., Sangchanjiradet, S., Yaraksa, N., & Aoki, S. (2022). Preliminary phytochemical screening and antioxidant activity of Dioscorea alata L. Advanced science journal, 22(2): R83 - R100. Retrieved from https://li02.tci-thaijo.org/index.php/adscij/article/view/400

Pang, Y., Ahmed, S., Xu, Y., Beta, T., Zhu, Z., Shao, Y., & Bao, J. (2018). Bound phenolic compounds and antioxidant properties of whole grain and bran of white, red and black rice. Food chemistry, 240, 212-221. https://doi.org/10.1016/j.foodchem.2017.07.095

Sen, S., Chakraborty, R., & Kalita, P. (2020). Rice - not just a staple food: A comprehensive review on its phytochemicals and therapeutic potential. Trends in food science & technology, 97, 265-285. https://doi.org/10.1016/j.tifs.2020.01.022

Settu, R., Selvaraj, D., & Padikasan, I. A. (2021). GCMS profiling and in silico screening of alpha-amylase inhibitors in traditional pigmented rice varieties (Oryza sativa Linn) of Tamil Nadu. Food bioscience, 42, 101154. https://doi.org/10.1016/j.fbio.2021.101154

Singleton, V.L., & Rossi, J.A., Jr. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphothungstic acid reagents. American journal of enology and viticulture, 16, 144-158. https://doi.org/10.5344/ajev.1965. 16.3.144

Toshima, S., Hirano, T., & Kunitake, H. (2021). Comparison of anthocyanins, polyphenols, and antioxidant capacities among raspberry, blackberry, and Japanese wild Rubus species. Scientia horticulturae, 285, 110204. https://doi.org/10.1016/j.scienta.2021.110204

Weyer, C., Bogardus, C., Mott, D.M., & Pratley, R.E. (1999). The natural history of insulin secretory dysfunction and insulin resistance in the pathogenesis of type 2 diabetes mellitus. The Journal of clinical investigation, 104(6), 787-794. https://doi.org/10.1172/JCI7231

Yawadio, R., Tanimori, S., & Morita, N. (2007). Identification of phenolic compounds isolated from pigmented rices and their aldose reductase inhibitory activities. Food chemistry, 101(4), 1616-1625. https://doi.org/10. 1016/j.foodchem.2006.04.016

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Published

2023-12-08

How to Cite

Khampa, P., Jankham, A. . ., Aoki, S. ., & Munglue, P. . (2023). Total Phenolic and Flavonoid Contents, Antioxidant Activity and Alpha-Amylase Inhibitory Activity of Four Local Rice Varieties . Science Journal, Chandrakasem Rajabhat University, 33(2), 157–168. retrieved from https://ph03.tci-thaijo.org/index.php/scicru/article/view/698

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Research Articles