ฤทธิ์ต้านเซลล์มะเร็งผิวหนังชนิดเมลาโนมาของสารสกัดเส้นใยเห็ดหลินจือเขากวางด้วยเอทานอล

Ananya ๋Jindaruk; Yaovapa Armsirirujiwet; Sunchai  Payungporn; Nattanan T-Thienprasert

Authors

Ananya ๋Jindaruk Department of Biochemistry, faculty of Science, Kasetsart University
Yaovapa Armsirirujiwet Department of Microbiology, faculty of Science, Kasetsart University
Sunchai Payungporn Department of Biochemistry, Faculty of Medicine, Chulalongkorn University
Nattanan T-Thienprasert Department of Biochemistry, faculty of Science, Kasetsart University

Keywords:

melanoma skin cancer, Ganoderma lucidum var. antler, apoptosis, gene expression

Abstract

Melanoma skin cancer is a cancer that has been found to have a significant increasing incidence every year. It is severe and accounts for up to 75% of deaths among all skin cancer patients. Current treatment approaches include surgery, chemotherapy, and radiation therapy associate with side effects on patients. Therefore, it is necessary to study and develop new treatment strategies. Previous reports showed that Ganoderma lucidum var. antler fruiting body extract have anticancer activities against various types of cancers, but there have been no reports on the anticancer effects on melanoma skin cancer. This study aimed to investigate the anticancer activity of G. lucidum var. antler mycelium extract with ethanol on melanoma skin cancer cells (A375). By using MTT assay, the results demonstrated that the mycelium extract increased toxicity against A375 cells in a dose dependent manner, but it had very low toxicity on normal cells (Vero). Moreover, the result from Annexin V assay showed that at 250 µg/mL of mycelium extract could induce apoptosis in A375 cells at a mild level. Additionally, the real-time polymerase chain reaction (PCR) indicated that at 250 µg/mL of the extract could downregulated the expression of the signal transducer and activator of transcription 1 (STAT1) gene, which is a marker gene for melanoma skin cancer, by up to five folds. However, it had no effect on the 2'- 5'- oligoadenylate synthetase 1 (OAS1) gene in A375 cells. Therefore, these experimental results present new knowledge, indicating that G. lucidum var. antler mycelium extract has potential for further in-dept study.

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