การหาปริมาณคาเฟอีนในเมล็ดกาแฟอาราบิก้าด้วยวิธีโครมาโทกราฟีของเหลวสมรรถนะสูง

Saowapa Chumanee; Ruchira Khoomsab

Authors

Saowapa Chumanee Corresponding author https://orcid.org/0000-0002-0077-620X
Ruchira Khoomsab

Keywords:

Coffee, Caffeine, Ultrasound-assisted extraction, High-Performance Liquid Chromatography, Diode-Array Detector

Abstract

Caffeine is an alkaloid organic compound. It has a stimulating effect on the central nervous system, often found in coffee beans. This research aimed to determine the caffeine content in Caffea Arabica L. beans using high-performance liquid chromatography with a diode array detector in the wavelength of 274 nm. The amount of caffeine in coffee beans was extracted by ultrasound-assisted extraction with methanol-water. The optimum separation was performed on the C8 column with isocratic elution consisting of methanol and water (30:70, v/v) as mobile phase at a 1 mL/min flow rate. The injection volume was 5 µl. In the evaluation of method validation, it was found that there was a linear relationship in the concentration range of 0.50 – 10.00 µg/mL (R² = 0.9999). The limit of detection and quantification were 0.13 and 0.43 µg/mL, respectively. The relative standard deviation percentage was 1.02-0.21%, while the percent recovery varied between 96.00 and 98.50%. The method was applied to determine the caffeine content in samples of roasted Arabica coffee beans grown in various locations. It was found that the caffeine content in the samples was in the concentration range of 1.10–1.49% w/w.

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