Application of Glyphosate as a Pre-Treatment for Mitosis Study in Helianthus annuus L.

Abstract

The objective of this studies was to find out the appropriated time for using a glyphosate solution for study of mitotic cell division using pretreatment technique. Completely Randomized Design (CRD) was set up with 3 replicates, 10 samples each of 4 treatments consist of control methods, 1 hour, 2 hours and 3 hours and using the Sunflower (Helianthus annuus L.) as example plant. The results indicated that the different of pretreatment with a glyphosate solution had effect on the percentage of inhibiting mitotic cell division in sunflowers at the confidence level of 95%. In addition, it was found that the pre-treatment method with glyphosate solution for 2 hours gave the highest percentage of inhibition of mitotic cell division in sunflowers, which was 65.00±5.00 percent. While the control method uses distilled water as a pre-treatment substance. It does not cause any inhibition of cell division (0 percent). Moreover, it was found that the scores for inhibiting mitotic cell division of sunflowers which caused by using glyphosate solution as a pre-treatment substance in all 3 treatments had values equal is 3 points. Therefore, in this research it is concluded that glyphosate concentration can be used as a pre-treatment substance for studying mitotic cell division in sunflowers.