Factors Influencing the Growth Rate for Cultivation of Chlorella sp.: A Case Study of Nakhon Si Thammarat College of Agriculture and Technology
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Abstract
The objective of this research was to compare the growth rate differences between Chlorella sp. cultured using the traditional method and pure Chlorella sp. cultivation and study the relationship between the number of Chlorella sp. cells and environmental factors. The traditional cultivation method uses inoculum from the Chlorella sp. production pond at Nakhon Si Thammarat College of Agriculture and Technology. The pure Chlorella sp. cultivation uses inoculum from the Songkhla Inland Fisheries Research and Development Center. The Chlorella sp. cultivation experiment was conducted in 1.5-liter plastic bottles under a light intensity of 2,000±500 lux, with a 24 hour light exposure period. A plant nutrient solution was provided with an initial electrical conductivity (EC) of 0.7 mS/cm and a pH of 6. The initial Chlorella sp. cell count was 3×10³ cells per milliliter (cell/ml). Chlorella sp. cell counts were recorded using haemacytometer. Data on air temperature, water temperature, relative humidity, pH, and EC were collected once daily for 20 days. The study found that Chlorella sp. from the pure inoculum average cell count was 1.716×107 cells/ml, significantly higher than the traditional cultivation method (p<0.05). The average pH was 7.40±0.97, the average EC was 0.63±0.05 mS/cm, and the average water temperature was 30.69±0.80 °C. The pH value showed a positive correlation with the number of Chlorella sp. cells, while water temperature, EC, air temperature, and relative humidity showed a negative correlation. Chlorella sp. from the traditional cultivation method showed the average cell count was 6.915×105 cells/ml, the average pH was 6.86±0.56, the average EC was 0.53±0.07 mS/cm, and the average water temperature was 30.59±1.51 °C. Water temperature, pH, and air temperature showed a positive correlation with the number of Chlorella sp. cells, while EC and relative humidity showed a negative correlation.
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