ไทย

Abstract

Cordycep militaris substrate had less content of bioactive compounds than the stroma, but contains carbohydrates, proteins, fats and minerals that can be processed into a variety of food products. This research is about the characteristics study of Cordycep militaris substrate, and effect of its extract on fermented food microbial growth for finding a solution to apply the Cordycep militaris substrate with fermented food. It was found that the physical characteristics of fresh substrate color was orange-yellow and had a musty smell similar to other mushroom substrates with 55.7 ± 2.2% moisture content. The dry substrate was reduced in weight ratio from fresh substrate to 2.2 ± 0.1 times. The substrate containing of carbohydrate, protein, fat, fiber, ash, calcium and phosphorus at 85.33 ± 0.37%, 8.89 ± 0.22%, 0.51 ± 0.12%, 2.19 ± 0.11%, 0.45 ± 0.03%, 0.05 ± 0.01% and. 0.08 ± 0.00%, respectively. Cordycepin and adenosine were found in the substrate at 420 and 119 mg/100 g, respectively. The main bioactive agent content in the substrate extracted with ethanol were higher than extracted with water by 50%. These characteristic results showed that Cordycep militaris substrate is suitable for formulation with the food composed of grains and alcohol which the orange-yellow color of the substrate does not affect the appearance. Therefore, microorganisms were isolated from rice wort, Khao Mak, wine, kefir, and tempeh. Three groups of microorganisms like yeast, mold, and bacteria were isolated. It was found that the extracts at concentrations of 2.5, 5 and 10% had no effect on growth of most yeast and mold, but partially inhibited growth of mold in the tempeh inoculum at 10% concentration, and inhibited bacterial groups at all test concentrations of the extract. So, Cordycep militaris substrate are suitable for fermented foods that use yeast and mold as key microorganisms.