Lignan Constituents and Pinoresinol-lariciresinol Reductase 3 Gene in Riceberry Rice
DOI:
https://doi.org/10.55164/jgrdi.v1i1.646Keywords:
Lignan, Riceberry, Pinoresinol - lariciresinol Reductase (PLR) Enzyme, OsPLR3 GeneAbstract
The objective of this research was to identify type and content of lignans in seed of Riceberry rice, stage of milk, dough and maturity, using HPLC (High Performance Liquid Chromatography) and to clone PLR gene encoding Pinoresinol - lariciresinol reductase, a key enzyme in lignan biosynthesis pathway. The secoisolaricinol (SECO) lignan was found, 1.70 ± 0.20, 1.96 ± 0.25 and 3.14 ± 0.19 mg/g, in milk, dough and maturity, respectively. The molecular structure of PLR homolog gene was 2,449 bps in length that comprised of 5 exons and 4 introns with 921 open reading frame (ORF) encoding 306 amino acids polypeptide. The Rossmann-fold NAD(P)(+)-binding proteins, a conserved domain for dehydrogenation was identified with a key amino acid, lysine, for active site. Expression analysis of OsPLR3 in the stages of seed development was performed. The lowest expression was found at milk and gradually inclined to highest expression at maturity stage. This showed correlation to SECO accumulation content during seed development. These suggest that SECO accumulation was probably controlled by OsPLR3 gene.
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