Preliminary Study of In vitro of Natural Trichoderma Fungi from Bamboo Leaves

Abstract

Trichoderma sp. AIR 1 was the natural strain isolated from bamboo leaves of Amphur Lomkao, Petchaboon province. The morphological characters of Trichoderma AIR 1 were studied. Its biocontrol potential against three soilborne fungal diseases : Fusarium oxysporum, Pythium aphanidermatum, and Phytophthora palmivora in vitro was analyzed on different solid medium [potato dextrose agar (PDA), banana peel agar (BPA) and carboxy methyl cellulose sodium salt congo red solid medium + 2 % banana peels (CMCc + 2 % BP)]. Trichoderma AIR 1 had globose to subglobose and smooth conidia. It inhibited the growth of 3 tested - pathogens. The least inhibition was observed in dual culture of P. aphanidermatum. Comparing these results with those of T. harzianum, Trichoderma AIR 1 was less effective in the suppression but did not differ significantly. Trichoderma AIR 1 was identified by PCR-based method by Biotechnology Research and Development Office. The result showed that the genetic pattern typical of T. erinaceum.