The Potential of Crude Extracts from Boletus griseipurpureus for Antimicrobial and Antioxidant Activities
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Abstract
This research aimed to evaluate the efficiency of crude extracts from Boletus griseipurpureus for antimicrobial activity, antioxidant activity and phytochemical screening of crude extracts. In addition, the total phenolic contents of the extracts were analyzed. Three crude extracts (methanol, dichloromethane and hexane extracts) were evaluated for antimicrobial and antioxidant activities using a colorimetric broth microdilution method and DPPH free radical scavenging assay, respectively. Seven pathogenic microorganisms were used in the antimicrobial tests, includingfour bacteria strains, Staphylococcus aureus ATCC25923, Methicillin resistant Staphylococcus aureus (MRSA) SK1, Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27853 and three yeast strains, Candida albicans ATCC90028, Candida albicans NCPF3153 and Cryptococcus neoformans ATCC90112.The results indicated that ethanol extract showed the strongest activity against yeasts, with MIC value of 512 µg/ml. The remaining extracts gave the antimicrobial activity against S. aureus, MRSA and C. neoformans with MIC value of 512 µg/ml. On the other hand, only ethanol extract had the best antioxidant activity with IC50 value of 15.5±0.02 µg/ml. Phytochemical screening of the extracts using standard protocols revealed the presence of phenolic compounds, alkaloids, saponins, and tannins. Furthermore, the total phenolic content determined by the folin–ciocalteu method was 11.23 ± 0.10 mg gallic acid equivalent per gram of extract.
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