ผลของ Modified Estradiol/Progesterone และ hCG เพื่อกำหนดเวลาผสมเทียมต่อสมรรถภาพทางการสืบพันธุ์ในแม่โคนม
Article Sidebar
Main Article Content
บทคัดย่อ
การศึกษานี้มีวัตถุประสงค์เพื่อศึกษาโปรแกรมเหนี่ยวนำการเป็นสัดด้วย estradiol (E2) progesterone (P4) ร่วมกับ hCG ในแม่โคนม ทำการศึกษาในแม่โคนมลูกผสมโฮลสไตน์ฟรีเชี่ยน จำนวน 15 ตัว แบ่งออกเป็นเป็น 3 กลุ่ม กลุ่มละ 5 ตัว กลุ่มที่ 1 E2 + P4, กลุ่มที่ 2 E2+P4+hCG และ กลุ่มที่ 3 E2+P4+Double Dose hCG วันที่ 0 ฉีด EB 2 ml (1 mg/ml) และสอดแท่ง P4 (CIDR) วันที่ 7 ฉีด PG 2 ml (0.25 mg/ml cloprostenol) และถอดแท่ง P4 โคกลุ่มที่ 2 ฉีด hCG 300 IU วันที่ 7 ในกลุ่มที่ 3 ฉีด hCG 300 IU เพิ่มวันที่ 8 และโคทั้ง 3 กลุ่ม วันที่ 8 ฉีด ECP 1 ml (1 mg/ml) และทำการผสมเทียมในวันที่ 10 จากการศึกษาไม่พบความแตกต่างทางสถิติ (P>0.05) ระหว่างกลุ่มทดลองในด้านลักษณะของมดลูก รังไข่ การแสดงการเป็นสัด ขนาดของ largest follicle (P=0.45) อัตราการตกไข่ (P=0.72) และขนาดของ CL (P=0.24) การศึกษาในครั้งนี้การเพิ่ม hCG ในโปรแกรมเหนี่ยวนำการเป็นสัด E2/P4 ไม่สามารถช่วยปรับปรุงความสมบูรณ์พันธุ์ของแม่โคนมเมื่อเทียบกับโคที่ไม่ได้รับ hCG
Article Details
เอกสารอ้างอิง
Bihon, A., Assefa, A., & González-Redondo, P. (2021). Prostaglandin based estrus synchronization in cattle: A review. Cogent Food & Agriculture, 7(1). https://doi.org/10.1080/23311932.2021.1932051
Burns, M. G., Buttrey, B. S., Dobbins, C. A., Martel, C. A., Olson, K. C., Lamb, G. C., & Stevenson, J. S. (2008). Evaluation of human chorionic gonadotropin as a replacement for gonadotropin-releasing hormone in ovulation-synchronization protocols before fixed timed artificial insemination in beef cattle. Journal of Animal Science, 86(10), 2539-2548. https://doi.org/10.2527/jas.2008-1122
Cardoso Consentini, C. E., Wiltbank, M. C., & Sartori, R. (2021). Factors that optimize reproductive efficiency in dairy herds with an emphasis on timed artificial insemination programs. Animals (Basel),11(2), 301. https://doi.org/10.3390/ani11020301
Cedeño, A. V., Cuervo, R., Tríbulo, A., Tríbulo, R., Andrada, S., Mapletoft, R., Menchaca, A., & Bó, G. A. (2021). Effect of expression of estrus and treatment with GnRH on pregnancies per AI in beef cattle synchronized with an estradiol/progesterone-based protocol. Theriogenology, 161, 294–300. https://doi.org/10.1016/j.theriogenology.2020.12.014
Colazo, M. G., Kastelic, J. P., Whittaker, P. R., Gavaga, Q. A., Wilde, R., & Mapletoft, R. J. (2004). Fertility in beef cattle given a new or previously used CIDR insert and estradiol, with or without progesterone. Animal Reproduction Science, 81(1-2),
-34. https://doi.org/10.1016/j.anireprosci.2003.09.003
Côrtes, L. R., Souza-Fabjan, J. M. G., Dias, D. S., Martins, B. B., Maia, A. L. R. S., Veiga, M. O., Arashiro, E. K. N., Brandão, F. Z., Oliveira, M. E. F., Bartlewski, P. M., & Fonseca, J. F. (2021). Administration of a single dose of 300 IU of human chorionic gonadotropin seven days after the onset of estrus improves pregnancy rate in dairy goats by an unknown mechanism. Domestic Animal Endocrinology, 74, 106579. https://doi.org/10.1016/j.domaniend.2020.106579
Cunha, T. O., Martinez, W., Walleser, E., & Martins, J. P. N. (2021). Effects of GnRH and hCG administration during early luteal phase on estrous cycle length, expression of estrus and fertility in lactating dairy cows. Theriogenology, 173, 23-31 https://doi.org/
1016/j.theriogenology.2021.06.010
da Silva, A. G., Nishimura, T. K., Rocha, C. C., Motta, I. G., Neto, A. L., Ferraz, P. A., &Pugliesi, G. (2022). Comparison of estradiol benzoate doses for resynchronization of ovulation at 14 days after timed-AI in suckled beef cows. Theriogenology, 184, 41-50.
De Rensis, F., Lopez-Gatius, F., Garcia-Ispierto, I., & Techakumpu, M. (2010). Clinical use of human chorionic gonadotropin in dairy cows: an update. Theriogenology, 73(8), 1001-1008. https://doi.org/10.1016/j.theriogenology.2009.11.027
Ferre, L. B., Cervino, M. N., Jaeschke, J., Itterman, M., McLean, J., Aragon, A., &de la Sota, R. L. (2025). Evaluation of GnRH- versus estradiol-based protocols for ovulation synchronization in postpartum Bos taurus grazing beef cows submitted to fixed-time artificial insemination. Theriogenology, 233, 1-7. https://doi.org/10.1016/j.theriogenology.2024.11.008
Garcia-Ispierto, I., De Rensis, F., Casas, X., Caballero, F., Mur-Novales, R., & Lopez-Gatius, F. (2018). Reproductive performance of lactating dairy cows after inducing ovulation using hCG in a five-day progesterone-based fixed-time AI protocol. Theriogenology, 107, 175-179. https://doi.org/10.1016/j.theriogenology.2017.11.012
Giordano, J. O., Edwards, J. L., Di Croce, F. A., Roper, D., Rohrbach, N. R., Saxton, A. M., &Schrick, F. N. (2013). Ovulatory follicle dysfunction in lactating dairy cows after treatment with Folltropin-V at the onset of luteolysis. Theriogenology, 79(8), 1210-1217. https://doi.org/10.1016/j.theriogenology.2013.02.020
Herzog, K., Brockhan-Ludemann, M., Kaske, M., Beindorff, N., Paul, V., Niemann, H., & Bollwein, H. (2010). Luteal blood flow is a more appropriate indicator for luteal function during the bovine estrous cycle than luteal size. Theriogenology, 73(5), 691-697. https://doi.org/10.1016/j.theriogenology.2009.11.016
Innes, D. J., Pot, L. J., Seymour, D. J., France, J., Dijkstra, J., Doelman, J., & Cant, J. P. (2024). Fitting mathematical functions to extended lactation curves and forecasting late-lactation milk yields of dairy cows. J Dairy Sci, 107(1), 342-358. https://doi.org/10.3168/jds.2023-23478
Pandey, A. K., Ghuman, S. P. S., Dhaliwal, G. S., Honparkhe, M., Phogat, J. B., & Kumar, S. (2018). Effects of preovulatory follicle size on estradiol concentrations, corpus luteum diameter, progesterone concentrations and subsequent pregnancy rate in buffalo cows (Bubalus bubalis). Theriogenology, 107, 57-62. https://doi.org/10.1016/j.theriogenology.2017.10.048
Pfeifer, L. F. M., Castro, N. A., Neves, P. M. A., Cestaro, J. P., & Schneider, A. (2017). Comparison between two estradiol-progesterone based protocols for timed artificial insemination in blocks in lactating Nelore cows. Animal Reproduction Science, 181, 125-129. https://doi.org/10.1016/j.anireprosci.2017.03.025
Prata, A. B., Drum, J. N., Melo, L. F., Araujo, E. R., & Sartori, R. (2018). Effect of different chorionic gonadotropins on final growth of the dominant follicle in Bos indicus cows. Theriogenology, 111, 52-55. https://doi.org/10.1016/j.theriogenology.2018.01.011
Rocha, C. C., Martins, T., Cardoso, B. O., Silva, L. A., Binelli, M., & Pugliesi, G. (2019). Ultrasonography-accessed luteal size endpoint that most closely associates with circulating progesterone during the estrous cycle and early pregnancy in beef cows. Animal Reproduction Science, 201, 12-21. https://doi.org/10.1016/j.anireprosci.2018.12.003
Rodriguez, A. M., Maresca, S., Lopez-Valiente, S., Bilbao, M. G., Moran, K. D., Bartolome, J. A., &Long, N. M. (2023). Comparison of the 7-day CO-Synch and 8-day estradiol-based protocols for estrus synchronization and timed artificial insemination in suckled Bos taurus cows. Theriogenology, 200, 70-76. https://doi.org/10.1016/j.theriogenology.2023.02.003
Sartori, R., Consentini, C. E. C., Alves, R., Silva, L. O., & Wiltbank, M. C. (2023). Review: Manipulation of follicle development to improve fertility of cattle in timed-artificial insemination programs. animal, 17 Suppl 1, 100769. https://doi.org/10.1016/j.animal.2023.100769
Taghizadeh, E., Barati, F., Fallah, A. A., Hemmatzadeh-Dastgerdi, M., & Nejabati, M. S. (2024). Estrogens improve the pregnancy rate in cattle: A review and meta-analysis. Theriogenology, 220, 35-42. https://doi.org/10.1016/j.theriogenology.2024.03.005
Valchuk, O. A., Kovpak, V. V., Kovpak, O. S., Salizhenko, M. I., Derkach, S. S., & Mazur, V. M. (2023). Concentration of progesterone in the blood serum and size of the corpus luteum as criteria for selection of recipient cows for embryo transfer. Regulatory Mechanisms in Biosystems, 14(4), 564-569. https://doi.org/10.15421/022382
Vynckier, L., Debackere, M., De Kruif, A., & Coryn, M. (1990). Plasma estradiol‐17ß concentrations in the cow during induced estrus and after injection of estradiol‐17ß benzoate and estradiol‐17ß cypionate‐a preliminary study. Journal of Veterinary Pharmacology and Therapeutics, 13(1), 36-42. https://doi.org/10.1111/j.1365-2885.1990.tb00745.x
Yanez, U., Murillo, A. V., Becerra, J. J., Herradon, P. G., Pena, A. I., & Quintela, L. A. (2023). Comparison between transrectal palpation, B-mode and Doppler ultrasonography to assess luteal function in Holstein cattle. Frontiers in Veterinary Science, 10, 1162589. https://doi.org/10.3389/fvets.2023.1162589
