Journal of Science and Technology Kasetsart University

Enhancement of Post-Thaw Sperm Quality and Reduction of Oxidative Stress in Bull Frozen Semen through Melatonin Supplementation in Egg Yolk-Tris Extender

Reanuka Somsrithanakorn — 2026-04-24

This study aimed to assess the impact of melatonin supplementation in egg yolk-tris extenders (ETG) on sperm quality and oxidative stress parameters in frozen semen of Kamphaeng Saen bulls. Semen samples were collected from 4 bulls. Each was sampled 4 times at one-week intervals. All collected semen samples were into 4 parts: a control group without melatonin supplementation and three treatment groups supplemented with melatonin in ETG at concentrations of 1, 2, and 3 mM. The findings demonstrated that melatonin supplementation at 1 and 2 mM significantly enhanced post-thawed sperm motility compared to both the control group and the 3 mM group (50.00, 51.51, 41.54, and 39.40%, respectively; P=0.0208). Additionally, the proportion of static sperm was significantly reduced in the 1 and 2 mM melatonin groups (P=0.0178). In terms of oxidative damage, assessed via malondialdehyde (MDA) levels, the 2 and 3 mM melatonin groups exhibited significantly lower MDA levels, compared to the control and 1 mM groups (1.0857, 1.1002, 2.8942, and 1.8061 µM, respectively; P<0.001). These findings suggest that melatonin supplementation at a concentration of 2 mM in ETG can enhance the quality of frozen-thawed bull semen by improving motility and reducing oxidative stress.

Prevalence of Blood Parasite Infections in Dogs and Cats in Samut Prakan and Surrounding Provinces, January–March 2024

Rapipan Siridet — 2026-04-24

Blood parasite infections in dogs and cats are a significant veterinary issue in Thailand. They often cause severe clinical symptoms and require specific treatments, particularly in cases of co-infections. This study aimed to investigate the prevalence of blood parasite infections in dogs and cats submitted for examination at The Unique Lab, Samut Prakan Province, between January and March 2024 using a thin blood smear technique. The prevalence of blood parasite infections in dogs was 29.42% of all dogs. The infections were identified as E. canis, A. platys, Babesia spp., H. canis and Trypanosoma spp., with prevalence rates of 25.00%, 1.33%, 0.44%, 0.22%, and 0.22% among the infected dogs, respectively. Co-infections of E. canis and A. platys were observed in 2.21% of infected dogs. In cats, Mycoplasma spp. infection was found in 3.45% of all cats. The results of this study provide baseline data essential for the diagnosis, control, and treatment of blood parasite infections in dogs and cats in Samut Prakan Province and surrounding areas.

Impact of Supplementation Fermented Juice from Mangifera Indica L. (Nam Dok Mai Sri Thong) On Performance, Ammonia Concentration in Manure, Egg Quality in Laying Japanese Quails and Chemical Composition of Quail Eggs

Pathama Thannark — 2026-04-24

Fermented juice of Mangifera indica L. (Nam Dok Mai Sri Thong) contains lactic acid, beta-carotene and beneficial microorganisms as a supplement that supports the quail production process. Therefore, this study investigated the potential benefits of supplementing the drinking water of laying Japanese quails with fermented juice of Nam Dok Mai Sri Thong. A total of 600 twenty-six-week-old female Japanese quails were used in this study. The birds were randomly distributed into three treatment groups according to a completely randomized design (CRD), with each group consisting of eight replications of 25 quails. The experimental groups were as follows: (1) a control group receiving only water (C); (2) a group supplemented with 1% fermented juice of Nam Dok Mai Sri Thong (FM 1%); and (3) a group supplemented with 3% fermented juice of Nam Dok Mai Sri Thong (FM 3%). The results showed that FM 1% and FM 3% effect on feed intake, water intake, hen-day production egg mass shape Index, shell weight, eggshell percentage, eggshell ratio, shell thickness and yolk color of quail eggs were significantly different (P<0.05) In contrast, there treatments did not affect feed per egg ratio, ammonia, egg weight yolk index, albumin index, albumin height, albumin weight, yolk weight, albumin ratio and yolk ratio were not significantly different (P>0.05). The chemical composition of quail eggs was not significantly different among the treatments (P>0.05).

Evaluation of Field User Satisfaction Toward a Robotic Arm-Equipped Drone for Fire Emergency Response

Punpaphatporn Bunprom — 2026-04-24

his study aimed to investigate the technical problems and limitations of unmanned aerial vehicles (UAVs) equipped with robotic arms for fire rescue missions in field contexts, as well as to assess the satisfaction of field users with this technology in actual operations. A quasi-experimental qualitative research design was employed as a pilot study. The sample consisted of 10 participants, including rescue personnel and occupational safety officers, selected through purposive sampling. Data were collected using questionnaires, interviews, and field trials in simulated scenarios. Quantitative data were analyzed using descriptive statistics, while qualitative data were examined through content analysis.

The findings revealed that users expressed the highest level of overall satisfaction with the technology (“strongly agree”; mean = 4.25±0.56), primarily because the robotic armed drone helped reduce risks to rescue personnel, provided rapid access to hazardous areas, and enabled timely responses to emergencies. The highest-rated dimension was “ease of use” (mean = 4.40±0.55), indicating that participants could quickly learn and operate the system without complexity. Identified technical limitations included difficulties in maneuvering within confined spaces, instability in grasping slippery or complex-shaped objects, limited flight duration due to battery capacity, and environmental impacts such as strong winds or rainfall.

The results suggest that robotic arm-equipped UAVs have significant potential to support rescue operations by enhancing efficiency, reducing risks to personnel, and enabling timely responses to emergencies. However, further development is recommended, such as integrating additional components (e.g., cameras mounted on the robotic arm, stabilization systems, and automatic emergency release functions), as well as establishing virtual training systems to strengthen effective use in real-world environments.

Digital System Development for Enhancing Cold Equipment Installation Management in Convenience Stores

Somnuek Suansawat — 2026-04-24

This study aims to improve the efficiency of refrigeration equipment installation management for convenience stores. The scope covers processes from job initiation, planning, resource allocation, and team coordination to the integration of digital systems. Information technology was introduced to enhance operational performance, improve service quality, reduce errors and time consumption, and establish a database to support future problem analysis and process improvement. The developed system was designed to record customer job orders, store relevant information such as customer data, installed equipment, installation sites, and technical specifications, while also enabling real-time status tracking.

The findings resulted in a prototype management system that allows retrospective data analysis, reduces paperwork, and minimizes manual workload in job monitoring. User evaluation indicated a satisfaction rate of 90%, confirming the system’s effectiveness in enhancing installation management efficiency and supporting continuous improvement in operational processes.

Production and Prediction of The Higher Heating Value of Corncob Biochar Using Machine Learning Techniques

Nopporn Rattanachoung — 2026-04-24

This study aims to enhance the quality of agricultural corncob biomass through the torrefaction process to improve its potential as a solid biofuel. The process was designed and simulated using Aspen Plus V12.1 and Design Expert V13.0, considering four main factors: biomass particle size, nitrogen gas flow rate, temperature, and reaction time. The response variable was the higher heating value (HHV). Experimental results revealed that the highest HHV of 19.3945 MJ/kg was obtained under the conditions of 20 mesh particle size, 30 mL/min nitrogen flow rate, 280°C, and 60 minutes, with an average deviation of 2.37%. Optimization using Design Expert indicated the optimal conditions at 57.60 mesh particle size, 27.40 mL/min nitrogen flow rate, 318.40 °C, and 66 minutes, yielding an HHV of 18.4853 MJ/kg. Machine learning techniques were employed to predict HHV, and the XGBoost (XGB) model demonstrated the highest prediction accuracy of 97.53%. Carbon content exhibited the strongest positive correlation with HHV, whereas oxygen and ash contents showed negative correlations. The results indicate that integrating process simulation with machine learning provides an effective approach to predict and optimize the torrefaction performance of corncob biomass, supporting the development of sustainable high-quality solid biofuel.

Development of Bioplastic from Corncobs to be a Guideline to Produce Biopolymers suitable for General Shopping Bags and Packaging Materials

Thanakrit Oonsaart — 2026-04-24

Polymers with many good properties, such as high durability, chemical resistance, and light weight, are a kind of convenience in our daily lives. However, these polymer materials, especially synthetic polymers rank the most environmental problems, taking 400-500 years or more to biodegrade. Another problem is that corncobs, also known as corn kernels, are the most common waste product of corn. Farmers tend to burn them, which creates smoke that damages the atmosphere, ecosystems, and public health in that area. It is estimated that 1.2 million tons of corncobs are wasted per year. Therefore, this project aims to study the properties of bioplastics from corncobs as a guideline for the production of suitable biopolymers for bags and packaging, using corn cobs as a starch source for bioplastics. Corn cobs, vinegar, glycerol, and water were boiled until they become thick. Then they were baked at 180 °C for 1 hour. After that, the properties such as tensile strength, density, water solubility and biodegradation of the three kind of plastic were tested. The results showed that bioplastic yield of 43.05 % w/w with the loss of 361.3 grams of solution. The obtained results showed that the bioplastic had high performance in the biodegradation test,was able to degrade more than 99% within 30 days. Thus can be concluded that the development of bioplastic is beneficial. However, solubility is limited.

Isolation and Assessment of Cellulase Activity in Actinobacteria from Herbivorous Animal Feces

Ratchanee Mingma — 2026-04-24

Animal feces serve as a habitat for diverse microorganisms, including bacteria, fungi, and protozoa, which are essential for breaking down organic matter, and contributing to nutrient cycle. Cellulose, a complex carbohydrate prevalent in plant cell walls, is abundant in herbivore feces and is broken down by cellulase-producing microorganisms that reside in their gut as well as in soil, decaying matter, and surrounding environment. The objective of this study was to isolate actinobacteria from herbivore faeces and evaluate their cellulase-producing potential. A total of 50 actinobacterial isolates were obtained from cow, horse, and goat faeces. Among these, 33 isolates produced clear zones on carboxymethyl cellulose (CMC) agar plates, with hydrolysis capacity (HC) values ranging from 2.0 to 7.2. Out of 33 isolates, 28 isolates exhibited measurable cellulase activity, ranging from 0.37 ± 0.022 U/ml to 0.98 ± 0.046 U/ml. The five isolates with the highest enzyme activity—MG08, MG15, MH01, MH08, MH09, and MH11—were identified as Streptomyces sp. and demonstrated the ability to tolerate temperatures up to 45 °C. These isolates represent promising candidates for further studies on cellulase production, with potential applications in biomass degradation, bioconversion of agricultural residues, and industrial enzyme production.

A Study of the Effects of Meditation Practice by Listening Chanting and Instrumental Music Affecting Training the Brain Ability

Chayanon Awikunprasert — 2026-04-24

This experimental research aimed to study the effects of meditation by listening to chanting and instrumental music on cognitive abilities through a sport intelligence program. The participants were 30 athlete students from Nakhon Phanom Provincial Sports School, divided into two purposive sampling groups, 15 students each using. An experimental design was divided 3 programs: athletic intelligence meditation practice using Buddies chants and meditation practice using music. Data were analyzed using t-tests with a significance level of .05.

Results showed no significant differences between groups before the intervention. After four weeks, the instrumental music group showed significant improvement in unique pattern design and spatial ability. Compared to the group Listening to chanting, participants performed significantly better in simple and choice reaction times, mental rotation, and spatial ability. The findings suggest that meditation, especially with instrumental music, can enhance cognitive processing and reaction time in athletes.

Assessment of Mental Toughness of High School Team Athletes in Nonthaburi Province

Eakarak Chaiyasatarn — 2026-04-24

This survey research aimed to (1) assess the level of overall mental toughness (MT) in high school student-athletes in Nonthaburi Province, and (2) compare the MT levels based on sport type and length of playing experience. The study utilized a purposive sample of 48 male athletes. Data were collected using the Thai version of the Psychological Performance Inventory (PPI). Statistical analysis included the mean, standard deviation, and Analysis of Variance The results indicated a high overall level of mental toughness among the athletes. However, no statistically significant differences were found when comparing mental toughness scores by sport type or length of playing experience (p > .05). The athletes' primary psychological strengths were Positive Energy and Attitude Control. Conversely, Attention Control and Negative Energy Control were identified as areas requiring development, scoring at a moderate level. These findings suggest that Psychological Skills Training program for this population should prioritize techniques for improving attention and managing negative energy to enhance performance under competitive pressure. Conversely, the components requiring development were Attention Control and Negative Emotion Control. Therefore, the Psychological Skills Training program for student-athletes should focus on developing Attention Control and negative emotion skills to enhance the athletes' ability to maintain their best possible performance state under competitive pressure.

Effects of Yoga Asana Training Program on Blood Glucose Levels and Muscle Mass in Individuals with Prediabetes

Nanthawan Thienkaew — 2026-04-24

This experimental study aimed to examine the effects of asana yoga training on blood glucose levels and muscle mass among individuals with hyperglycemia. The sample consisted of 31 participants, divided into an experimental group (n=16) and a control group (n=15). The asana yoga training program wasvalidated for content validity with an overall IOC value of 0.83. Assessments were conducted at 8 and 12 weeks, including muscle mass measurement using Dexa Scan, blood glucose parameters, and leg muscle strength testing using an isokinetic device. The results revealed no statistically significant differences in blood glucose levels between the groups (p > .05); however, the experimental group showed smaller increases in glucose levels compared to the control group. Notably, leg flexion strength in the experimental group significantly improved (p = 0.045), and total muscle mass demonstrated a tendency to increase over the long term. In conclusion, asana yoga training contributes to muscle mass enhancement and may promote better glucose utilization, although blood glucose
levels did not significantly decrease within the 12-week intervention period.

Development of Safety Practice Guidelines for the Installation and Maintenance of Rooftop Solar Power Systems Based on Systemic Risk Assessment Using What-If Analysis Technique for Thailand

Patiya Ploynoy — 2026-04-24

Electricity generation from rooftop solar systems is one of the key approaches to promoting renewable energy in Thailand. However, the installation and maintenance of such systems pose safety risks to workers, particularly from falls from height, electric shocks, and falling objects. This study aims to examine relevant safety standards both domestically and internationally, identify gaps in Thai standards, and assess risks using the What-If Analysis technique in order to develop appropriate safety guidelines. 14 standards and manuals from the United States, the United Kingdom, Australia, New Zealand, and Thailand and a risk assessment framework based on 10 key issues were analyzed. The risk assessment results revealed that 93% of the case scenarios were at an unacceptable risk level. Based on these findings, a practical safety guideline was developed for the installation and maintenance of rooftop solar systems, along with a pre-work safety checklist that can be effectively applied in practice, tailored to Thailand’s actual working conditions and constraints.

Development and Performance Evaluation of an ATV-Mounted Boom Sprayer for Weed Control in Large Agricultural Areas

Teerawat Chuenatsadongkot — 2026-04-24

This research aimed to develop and evaluate the performance of a prototype herbicide sprayer designed for mounting on All-Terrain Vehicles (ATVs), commonly used in fruit orchards. The development addressed weed management challenges in large-scale durian orchards of a community enterprise in Chanthaburi province, focusing on issues of labor cost, efficiency, and chemical application safety. The prototype consisted of a 20-liter electric spray tank and a 1-meter-wide spray boom constructed from PVC conduit, fitted with five mist nozzles. Field tests were conducted on a 1-rai (1,600 m²) plot using a 125 cc ATV operating at an average speed of 3-5 km/h. The performance test results of the developed sprayer set showed that it could operate in a real setting with an average spraying time of just 19.77 minutes per rai. and an ATV fuel consumption rate of 0.33 liters per rai. Compared to the conventional backpack spraying method, the new system reduced operational time by over 60% and total operational costs (fuel and labor) by 62.3%. It also increased the daily work capacity from 4 rai to 10 rai and significantly enhanced operator safety by minimizing direct chemical exposure. Therefore, the developed ATV-mounted sprayer represents an appropriate, highly efficient, cost-effective, and safer technology for farmers in large-scale agricultural settings.

Comparison of Piezometric Head Relationships Using Statistical Analysis: A Case Study of Pasak Jolasit Dam

Peerapon Putipen — 2026-04-24

The analysis of data from dam behavior monitoring instruments provides an understanding of the behavior occurring within the dam. Piezometers are widely used instruments to monitor seepage and internal erosion in earth dams. This study analyzes the data using three statistical methods: Simple Linear Regression (SLR), Multiple Linear Regression (MLR), and Dummy Variable Regression (DVR), to examine the relationships between piezometric head, reservoir water levels, and related factors, as well as to identify the most appropriate analytical method. The results show that SLR provides correlation coefficients (R) ranging from 0.05 to 0.70, MLR from 0.26 to 0.72, and DVR from 0.43 to 0.81. SLR is a basic and convenient method but yields the lowest correlations, and is suitable to explain the behavior of upstream piezometers. MLR provides higher correlations, especially for piezometers influenced by rainfall, but it suffers from multicollinearity. DVR, which analyzes the relationships based on temporal variations in reservoir water levels, is the most appropriate method, providing the highest correlations, reducing multicollinearity, and yielding the best AIC and BIC values. However, the suitability of each analytical method should depend on the location of the piezometers and correspond to the current behavioral characteristics.

Assessment of NASA POWER Satellite Rainfall Product to Predict Inflow at Naruebodindrachinta Reservoir

Teerawat Senahan — 2026-04-24

The rainfall observation data in the upstream catchment of the Naruebodindrachinta Reservoir is limited. The use of satellite rainfall products has become an important alternative to enhance the accuracy of reservoir inflow estimation. This study aimed to evaluate the performance of NASA POWER satellite rainfall data to analyze the monthly inflow to the Naruebodindrachinta Reservoir using the NAM rainfall–runoff model. The study was divided into two scenarios—before and after the reservoir construction—to compare the differences in model parameters and performance efficiency. The calibration results indicated that the correlation coefficient (r) and the Nash–Sutcliffe efficiency (NSE) were at a good level, particularly after the reservoir construction, with r = 0.87 and NSE = 0.69. The model verification in 2021 yielded r = 0.81 and NSE = 0.65, while the prediction for 2024 achieved r = 0.98 and NSE = 0.91. These results demonstrate that the combination of NASA POWER satellite rainfall data with the NAM model can accurately simulate the reservoir inflow. The finding highlights the potential of NASA POWER satellite rainfall data to support sustainable water resources management and serve as a guideline to develop runoff prediction models in data-scarce basins.