Effect of Plant Growth Regulators on Cape Gooseberry In vitro Culture

Abstract

The effect of Plant Growth Regulators on Cape Gooseberry was conducted through Sodium
hypochlorite. The results revealed that an optimal sterilizing condition was soaking in 15% Sodium
hypochlorite for 15 min. This sterilizing condition gave the lowest microbial contamination rate at 10 % and
the highest plant survival rate at 90 %. Induction of callus from young leaf explants was investigated. The
result showed that MS medium supplemented with the combination of 0.5 mg/l BA and 0.3 mg/l NAA was
induced the highest callus induction rate at 100% and the largest callus diameter (1.56±0.02 cm). Multiple
shoot induction from shoot tips were investigated. The result showed that MS medium supplemented with
the combination of 3.0 mg/l Kn and 0.1 mg/l NAA was the maximum number of shoots (7.6±0.40 shoots per
explants), average length of shoot (2.34±0.23 cm) and average number of leaves (6.80±0.37 leaves per
shoot). The plantlets were acclimatized and planted in greenhouse with the 100% survival rate. Suggesting
that callus could extract bioactive phytochemicals for further medical utilization.