Tissue Culture of Rosa chinensis Jacq. var. minima Voss. by Simple Plant Tissue Culture Media

Abstract

The objective of this study was to study effect of simple media on miniature
roses (Rosa chinensis Jacq. var. minima Voss.) shoot induction by a simple technique.
The node explants were washed by dishwashing liquid for 5 minutes before being
sterilized with 0.5 ml/L of 15% Haiter containing dishwashing liquid for 25 minutes
following by 5 minutes of sterilized water for 3 times. Sterilized explants were cultured
on 1/2 MS medium supplemented with 0.5ml/L Haiter for 2 weeks. After that, the
explants were cultured for 6 weeks on MS medium and simple media prepared from 5
ml/L Hydroponics A and B that supplemented with various concentrations of BA at 0, 1,
3 and 5 mg/L. Then, shoot number, leaf number and shoot length were observed and
recorded. The result showed that a simple medium supplemented with 3 mg/L BA
displayed the highest average of shoot and leaf number of 1.33 ± 0.68 and 5.96 ± 3.09,
respectively. Furthermore, the highest average of shoot length was 1.57 ± 0.86 cm for
MS medium with the statistically significant difference from other simple media
whereas simple media without BA showed average of shoot length without significant
differences from simple media supplemented with 1, 3 and 5 mg/L BA. This research
provides a basis for further study in low-cost miniature roses propagation.