Isolate the Biosurfactant-producing bacteria from soil for washing and bioremediation of environmentally contaminated oil

Abstract

The purpose of this study was to isolate the Biosurfactant-producing bacteria from soil contaminated with crude oil or its byproducts in Uttaradit Province. A total of 7 bacterial strain was cultured in the mineral salt medium at 30 o C for 5 days. The Emulsification activity test (EA24) were exploited in order to assess the efficiency of biosurfactant production after cultured period. Among these isolates, three of them including isolates 4, 7 and 6 produced the highest number of biosurfactant (EA24) at 40.74, 35.00and27.78 %, respectively. The Fermentation broth from isolates 4, 7 and 6 were further investigated for oil removal potential by sand pack test, oil recovery using de emulsification activity and emulsion property using emulsion stability. Interestingly, the results showed that the oil removal potential of 20 % (v/v) at 24.52, 27.78and18.71%, respectively. With 16s rRNA analysis the isolate 4 was identified as Bacillus sp.