Antimicrobial Activity of Medicinal Plant and Edible Mushroom Crude Extracts

Abstract

The purpose of this research is to investigate the antimicrobial activity of 11 medicinal plantand 4 edible mushroomcrude extracts.Medicinal plants and mushrooms were macerated in99.9%methanol for extraction.Then evaporated the solvent with a rotary vacuum evaporator. After that, crude extracts were antimicrobial activities tested of 9 test microorganisms including Staphylococcus aureus, S. epidermidis, Bacillus cereus, B. subtilis, Escherichia coli, Pseudomonas aeruginosa, P. fluorescens,Vibrio parahaemolyticus and Candida albicans using agar well diffusion method. The results revealed that crude extracts from Boesenbergia rotunda (Linn.) Mansf. exhibited the highest inhibition zone on B. cereus andB. subtilisat 19.33±1.15 and 19.67±5.03 mm, respectively. The crude extract from Alpinia galanga (Linn.) Willd. exhibited the highest inhibition zone on S. aureusand S. epidermidisat 36.67±0.58 and35.33±0.58 mm, respectively. The inhibitory activity was higher than tetracycline (30 μg/mL) with clear zone of 28.00±3.46 and 19.33±1.52 mm, respectively. Moreover, the extract of Alpinia galanga (Linn.) Willd. showed the highest inhibition zone to C. albicans at 20.00±0.00 mm.While, the crude extract from Volvariella volvacea Fr. showed the highest inhibition zone on S.aureusand P. fluorescensat 15.67±1.53 and15.00±1.00 mm, respectively.The crude extract from Pleurotus djamor (Rumph. ex Fr.) Boedijnexhibited the highest inhibition zone on C. albicansat 11.33±0.58 mm.Therefore, the medicinal plant and edible mushroom extracts from this research may be efficaciously used as alternatives to replace of chemical agents in the further.