Effect of Murashige and Skoog (1962) Medium in Combination with Hydroponics Fertilizer RSU S1 for Cannabis Tissue Culture

Abstract

The effects of modifying Murashige and Skoog’s (MS) medium with hydroponics fertilizer on cannabis (Cannabis sativa L.subsp. indica) tissue culture using nodal segments were investigated.  MS media was supplement with four different nutrient levels hydroponics fertilizer including  ½ MS + hydroponics RSU S1 200 ppm, ½ MS + hydroponics RSU S1 300 ppm, MS + hydroponics RSU S1 200 ppm, and MS + hydroponics RSU S1 300 ppm, in comparison with MS as the control treatment.  Explants were cultured on each treatments for eight weeks under fluorescent illumination 16 hours photoperiod per day at 25±2 °C. The result revealed that MS + hydroponics RSU S1 300 ppm and MS + hydroponics RSU S1 200 ppm triggered the greatest increase in leaves (30.875 and 30.664 leaves) and canopy width (3.382 and 3.229 cm). MS medium with hydroponics RSU S1 300 ppm produced the best shoot length (2.443 cm) , fresh weight (0.896 g), and dry weight (0.113 g). The most shoot were detected in ½ MS + hydroponics RSU S1 300 ppm (6.739 shoots), however the percentage of root formation and survival rate were not statistically significant.