Daeng Ubon Orchid Plant Tissue Culture Technology for the Conservation of Native Orchid Dendrobium 'Daeng Ubon'

Doritis pulcherrima Var. buyssoniana, commonly known as “Daeng Ubon Orchid,” is a native orchid species endemic to Ubon Ratchathani Province, Thailand.

Abstract

Doritis pulcherrima Var. buyssoniana, commonly known as “Daeng Ubon Orchid,” is a native orchid species endemic to Ubon Ratchathani Province, Thailand. Due to habitat destruction, climate change, and human activities, the species is under threat and at risk of extinction. Tissue culture technology offers an effective conservation approach, enabling rapid and large-scale propagation. This process begins with the collection and surface sterilization of seed pods post-pollination. The seeds are then cultured on a modified Vacin and Went medium under aseptic conditions. Over time, the seeds develop into protocorms, seedlings, and eventually robust young plants that can be transplanted in greenhouses and returned to natural habitats. Additionally, the growth and development of D. pulcherrima Var. buyssoniana are influenced by several environmental factors including light intensity, temperature, humidity, air circulation, and suitable growing media. Simulating natural habitat conditions enhances plant growth and flowering quality. Micropropagation through tissue culture represents a sustainable and practical method for the conservation of D. pulcherrima Var. buyssoniana, contributing to the long-term protection of Thailand’s unique plant biodiversity.