Efficacy of Bacillus atrophaeus strain RS36 and Priestia megaterium strain RS91 with Partially Reduced Fertilization for Bacterial Leaf Blight Suppression in Rice Seedlings

Kanchalee Jetiyanon; Sasiwimon Boontawee; Suttita Padawan; Pinyupa Plianbangchang

doi:10.69650/ahstr.2024.2509

Authors

Kanchalee Jetiyanon Department of Agricultural Sciences, Faculty of Agriculture Natural Resources, and Environment, Naresuan University, Phitsanulok, 65000 Thailand
Sasiwimon Boontawee Department of Agricultural Sciences, Faculty of Agriculture Natural Resources, and Environment, Naresuan University, Phitsanulok, 65000 Thailand
Suttita Padawan Department of Agricultural Sciences, Faculty of Agriculture Natural Resources, and Environment, Naresuan University, Phitsanulok, 65000 Thailand
Pinyupa Plianbangchang 80 Soi Sukhumvit 40, Sukhumvit Road, Phra Kanong, Klong Toei, Bangkok 10110 Thailand

DOI:

https://doi.org/10.69650/ahstr.2024.2509

Keywords:

plant growth-promoting rhizobacteria, induced systemic resistance, bacterial leaf blight, reduced fertilization

Abstract

Four plant growth-promoting rhizobacteria (PGPR) strains; Bacillus atrophaeus strain RS36, Priestia koreensis strain RS86, Priestia megaterium strain RS91 and B. macauensis strain RS100, were previously reported for their growth enhancement and anthracnose disease reduction in peppers. RS36 and RS86 do not produce siderophore, while RS91 and RS100 do. There is little evidence of using PGPR-mediated induced systemic resistance with reduced fertilization to control bacterial leaf blight (BLB) in rice seedlings. This study aimed to investigate the efficacy of those four individual PGPR strains and their co-inoculation with 75% recommended chemical fertilizer rate (RFR) against BLB disease in rice seedlings. Non-siderophore-producing PGPR strains experiment and siderophore-producing PGPR strains experiment were tested separately. Each experiment was conducted twice and contained a single strain and their mixtures with 75%RFR. Nonbacterized treatment with 100%RFR served as a control in each experiment. A completely randomized design was set up with 4 replications per treatment. Results demonstrated that rice seedlings treated with a single PGPR strain and their mixtures with 75% RFR generally had a lower percentage of disease severity than rice seedlings in the control treatment. Nevertheless, only rice seedlings treated with a single strain of RS36 in the non-siderophore PGPR experiment and a single strain of RS91 in the siderophore-producing PGPR experiment provided a significantly lower percentage of disease severity (P£0.05) than the control of each experiment. No synergistic effect of disease suppression occurred when using PGPR mixtures. In conclusion, certain individual PGPR strains together with reduced fertilizer amount significantly suppressed BLB disease in rice seedlings.

References

Adesemoye, A. O., Tobert, H. A., & Kloepper, J.W. (2009). Plant growth-promoting rhizobacteria allow reduced application rates of chemical fertilizers. Microbial Ecology, 58, 921–929. https://doi.org/10.1007/s00248-009-9531-y

Ahsan, R., Ullah, S., Yaseen, I., Fateh, F. S., Fayyaz, M., Asad, S., Jamal, A., Sufyan, M., & Zakria, M. (2021). Assessment of bacterial leaf blight incidence and severity in rice growing areas of Pakistan. Pakistan Journal of Agricultural Research, 34(4), 693-699. https://dx.doi.org/10.17582/journal.pjar/2021/34.4.693.699

Ali, S. A. M., Sayyed, R. Z., Mir, M., Khan, M. Y., Hameeda, B., Alkhanani, M. F., Haque, S., Tawada, M. A., & Poczai, P. (2022). Induction of systemic resistance in maize and antibiofilm activity of surfactin from MS20. Frontiers in Microbiology, 13, 879739. https://doi.org/10.3389/fmicb.2022.879739

Batool, S., & Altaf, M. A. (2017). Plant growth promoting rhizobacteria (PGPR) reduces application rates of fertilizers in chilli (Capsicum frutescens L.) cultivation. Journal of Horticulture, 4(4), 1000215. https://doi.org/10.4172/2376-0354.1000215

Beijerinck M.W. (1898) Über ein contagium vivum fluidum als ursache der fleckenkrankheit der tabaksblätter. Verhandelingen der Koninklijke Akademie van Weterschappen, Afdeeling Natuurkunde, 6:3–21.

De Vleesschauwer, D., Cornelis, P., & Höfte, M. (2006). Redox- active pyocyanin secreted by Pseudomonas aeruginosa 7NSK2 triggers systemic resistance to Magnaporte grisea but enhances Rhizoctonia solani susceptibility in rice. Molecular Plant Microbe Interaction, 19(12), 1406−1419. https://doi.org/10.1094/MPMI-19-1406

Duy, P. N., Lan, D. T., Thu, H. P., Thu, H. P. T., Thanh, H. N., Pham, N. P., Auguy, F., Thu, H. B. T., Manh, T. B., Cunnac, S., & Pham, X. H. (2021). Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter, PLoS ONE, 13(3), 1-16. https://doi.org/10.1371/journal.pone.0255470

Hop, D. V., Phuong Hoa, P. T., Quang, N. D., Ton, P. H., Ha, T. H., Hung, N. V., Van, N. T., Hai, T. V., Kim Quy, N. T., Anh Dao, N. T., & Thom, V. T. (2014). Biological control of Xanthomonas oryzae pv. oryzae causing rice bacterial blight disease by Streptomyces toxytricini VN08-A-12, isolated from soil and leaf-litter samples in Vietnam. Bicontrol Science, 19(3), 103-111. https://doi.org/10.4265/BIO.19.103

Jetiyanon, K., & Kloepper, J. W. (2002). Mixtures of plant growth-promoting rhizobacteria for induction of systemic resistance against multiple diseases. Biological Control, 24(3), 285-291. https://doi.org/10.1016./S1049-9644(02)00022-1

Jetiyanon, K., & Wittaya-areekul, S. (2009). Application of rhizobacteria for induced disease resistance against anthracnose, bacterial wilt, and southern blight diseases in pepper Research report. Thailand Research Fund.

Jetiyanon, K., & Plianbangchang, P. (2012). Potential of Bacillus cereus strain RS87 for partial replacement of chemical fertilisers in the production of Thai rice cultivars. Journal of the Science of Food and Agriculture, 92(5), 1080-1085. https://doi.org10.1002/jsfa.5533

Jetiyanon, K., & Plianbangchang, P. (2013). Lipopolysaccharide of Enterobacter asburiae strain RS83: A bacterial determinant for induction of early defensive enzymes in Lactuca sativa against soft rot disease. Biological Control, 67(3), 301-307. https://doi.org/10.1016/j.biocontrol.2013.09.014

Ke, Y., Hui, S., & Yuan, M. (2017). Xanthomonas oryzae pv. oryzae inoculation and growth rate on rice by leaf clipping method. Bio-Protocol, 7(19), 1-7. https://doi.org/10.21769/BioProtoc.2568

Li, C., Zhou, L., Wu, B., Li, S., Zha, W., Li, W., Zhou, Z., Yang, L., Shi, L., Lin, Y., & You, A. (2022). Improvement of bacterial blight resistance in two conventionally cultivated rice varieties by editing the noncoding region. Cells, 11(16), 2535. https://doi.org/10.3390/cells11162535.

Meziane, H., Van der Sluis, I., Van Loon, L. C., Höfte, M., & Bakker, P. A. H. M. (2005). Determinants of Pseudomonas putida WCS358 involved in inducing systemic resistance in plants. Molecular Plant Pathology, 6(2), 177−185. https://doi.org/10.1111/j.1364-3703.2005.00276.x

Nasir, M., Iqbal, B., Hussain, M., Mustafa, A., & Ayub, M. (2019). Chemical management of bacterial leaf blight disease in rice. Journal of Agricultural Research, 57(2), 93-98.

Pacific Pests & Pathogens. (2019). Rice bacterial leaf blight (418). https://apps.lucidcentral.org/ppp/text/web_full/entities/rice_bacterial_leaf_blight_418.html On 17 October 2023

Rahma, H., Nurbailis, & Kristina, N. (2019). Characterization and potential of plant-growth promoting rhizobacteria on rice seedling growth and the effect on Xanthomonas oryzae pv. oryzae. Biodiversitas Journal of Biological Diversity, 20(12), 3654-3661. https://doi.org/10.13057/biodiv/d201226

Rajer, F. U., Samma, M. K., Ali, Q., Rajar, W. A., Wu, H., Raza, W., Xie, Y., Tahir, H. A. S., & Gao, X. (2022). Bacillus spp.-mediated growth promotion of rice seedlings and suppression of bacterial blight disease under greenhouse conditions. Pathogens, 11(11), 1251. https://doi.org/10.3390/pathogens11111251

Reddy, A. P. K., Katyal, J. C., Rouse, D. I., & MacKenzie, D. R. (1979). Relationship between nitrogen fertilization, bacterial leaf blight severity, and yield of rice. Phytopathology, 69(9), 970-973. http://doi.org/10.1094/Phyto-69-970

Ryu, C. M., Farag, M. A., Hu, C. H., Reddy, M. S., Kloepper, J. W., & Paré, P. W. (2004). Bacterial volatiles induce systemic resistance in Arabidopsis. Plant Physiology, 134(3), 1017−1026. https://doi.org/10.1104/pp.103.026583

Salvo, L. P. Di., & Salamone, I. E. G. de. (2019). Veil-like pellicle development by Azospirillum brasilense in semisolid NFb medium. Revista Argentina de Microbiología, 51(2), 184-185. https://doi.org/10.1016/j.ram.2018.04.002

Sharma, P., Baidya, S., Kandel, S., Chaudhary, S., & Magar, P. B. (2022). Management of bacterial leaf blight disease of rice in farmer’s field condition at Bhaktapur district of Nepal. Journal of Agriculture and Natural Resources, 5(1), 105-112. https://doi.org/10.3126/janr.v5i1.50646

Van Loon, L. C., Bakker, P. A. H. M., & Pieterse, C. M. J. (1998). Systemic resistance induced by rhizosphere bacteria. Annual Review of Phytopathology, 36, 453-483. https://doi.org/10.1146/annurev.phyto.36.1.453

Wang, S., Liu, W., Lu, D., Lu, Z., Wang, X., Xue, J., & He, X. (2020). Distribution of bacterial blight resistance genes in the main cultivars and application of Xa23 in rice breeding. Frontiers in Plant Science, 11, 1-10. https://doi.org/10.3389/fpls.2020.555228

Weller, D. M, Mavrodi, D.V., Van Pelt, J. A., Pieterse, C. M. J., Van Loon, L. C., & Bakker, P. A. H. M. (2012). Induced systemic resistance in Arabidopsis thaliana against Pseudomonas syringae pv. tomato by 2,4 Diacetylphloroglucinol-producing Pseudomonas fluorescens. Phytopathology, 102(4), 403-412. https://doi.org/10.1094/PHYTO-08-11-0222

Yasmin, S., Zaka, A., Imran, A., Zahid, M. A., Yousaf, S., Rasul, G., Arif, M., & Mirza, M. S. (2016). Plant growth promotion and suppression of bacterial leaf blight in rice by inoculated bacteria. PLoS One, 11(8), 1-19. https://doi.org/10.1371/journal.pone.0160688

Yasmin, S., Hafeez, F. Y., Mirza, M. S., Arshad, H. M. I., Zubair, M., & Iqbal, M. (2017). Biocontrol of bacterial leaf blight of rice and profiling secondary metabolites produced by rhizospheric Pseudomonas aeruginosa BRp3. Frontiers in Microbiology, 8, 1895. https://doi.org/10.3389/fmicb.2017.01895