Potential of Medium-Chain Glycerides-Based Microemulsions for Provoking Immune Response
Keywords:
microemulsions, immune, oralAbstract
Medium-chain glycerides-based microemulsions have been demonstrated to enhance the intestinal absorption of some peptides. Thus, this may be beneficial for using as an antigen delivery system. Therefore, the present study was aimed to investigate the potential of a medium-chain glycerides-based w/o microemulsion for provoking immune response following oral administration using Balb/c mouse as a model. The microemulsion was prepared by mixing 76% of oil mixture (Crodamol GTCC:Capmul MCM 3:1), 14% of surfactant mixture (polysorbate 80:sorbitan mono-oleate, 3:2) and 10% water. Model antigen, ovalbumin (OVA), 50 mg was dissolved in 1 ml of the aqueous fraction of the microemulsion. The ability to elicit immune responses of the microemulsion containing OVA was investigated by administering orally for three consecutive days at four weeks after the mice were primed subcutaneously with nanoparticles containing OVA. The results were demonstrated that the medium-chain glycerides-based microemulsion provoked higher immune responses, compared to OVA solution, suggesting that the microemulsion prepared may be a useful delivery system for an antigen. However, the responses were not maintained for long period.
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