Comparison of Various Extraction Methods of Bacopa monnieri

Watoo Phrompittayarat; Waraporn Putalun; Hiroyuki Tanaka; Kanchalee Jetiyanon; Sakchai Wittaya-areekul; Kornkanok Ingkaninan

Authors

Watoo Phrompittayarat Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University,Phitsanulok 65000, Thailand; Cosmetics and Natural Products Research Center, Naresuan University, Phitsanulok 65000, Thailand
Waraporn Putalun Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
Hiroyuki Tanaka Department of Medicinal Plant Breeding, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka812-8582, Japan
Kanchalee Jetiyanon Department of Agricultural Sciences, Faculty of Agriculture Natural Resources and Development, Naresuan University,Phitsanulok 65000, Thailand
Sakchai Wittaya-areekul Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000,Thailand.
Kornkanok Ingkaninan Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University,Phitsanulok 65000, Thailand.Cosmetics and Natural Products Research Center, Naresuan University, Phitsanulok 65000, Thailand

Keywords:

Bacopa monnieri, Triterpenoid saponins, Extraction method, High performance liquid chromatography

Abstract

Bacopa monnieri (L.) Wettst. (Brahmi) has been used in Ayurvedic medicine as nervine tonic for promoting mental health and improving memory brain function. Tetracyclic triterpenoid saponins were reported to be the responsible molecules for cognition enhancement. In order to optimize the extraction methods of Brahmi for commercial purpose, nine different methods were compared. Five major saponins i.e. bacoside A₃, bacopaside II, bacopasaponin C isomer, bacopasaponin C and bacopaside I in the extracts were determined using HPLC technique and calculated as total amounts of saponins. Among nine methods, the highest yield (27.89+0.48 %) was found in the extract obtained from maceration of the plant material in methanol at room temperature for 3 days. However, the extract containing the highest amount of total saponins (19.28+ 0.12 %) was obtained from percolation with ethanol after the plant material was soaked in water.