Solid Lipid Microparticles as a Delivery Carrier for Turmeric Crude Extract Containing Curcuminoids

Waree Tiyaboonchai; Premnapa Srisopa; Jarupa Viyoch; Kornkanok Ingkaninan

Authors

Waree Tiyaboonchai
Premnapa Srisopa
Jarupa Viyoch
Kornkanok Ingkaninan

Keywords:

Turmeric crude extract, Curcuminoids, Solid lipid microparticles, Hot emulsion technique

Abstract

Solid lipid microparticles comprising mixtures of stearic acid, glyceryl monostearate and medium chain triglyceride and containing turmeric crude extract were successfully developed in a one-step procedure, using a hot emulsion technique followed by high speed homogenization. A yellowish cream with a plastic flow behavior and thixotropic characteristics was obtained. Light microscopic investigations revealed that the turmeric extract loaded solid lipid microparticles possessed a narrow size distribution, ranging from 1-7 µm with high entrapment efficacy up to 100%. Dissolution studies using Franz diffusion cells demonstrated a fast release behavior suggesting that the curcuminoids were adsorbed on the particle surface. The stability of curcuminoids in the solid lipid microparticles could be maintained for at least 6 months in the lipidic preparation formulated together with Tween 80.

References

Ahsan, H., Parveen, N., Khan, N. U., & Hadi, S. M. (1999). Pro-oxidant, anti-oxidant and cleavage activities on DNA of curcumin and its derivatives demethoxycurcumin and bisdemethoxycurcumin. Chemico-BiologicalInteractions,121, 161-175.

Bemabe-Pineda, M., Ramirez-Silva, M. T., Romero-Romo, M. A., Gonzalez-Vergara, E., & Rojas-Hernandez, A. (2004). Determination of acidity constants ofcurcumin in aqueous solution and apparent rate constant of its decomposition. Spectrochimica Acta-Part A. Molecular and Biomolecular Spectroscopy, 60, 1091-1097.

Cortesi, R., Esposito, E., Luca, G., & Nastruzzi, C. (2002). Production oflipospheres as carriers for bioactive compounds. Biomaterials, 23, 2283-2294.

Jaspart, S., Bertholet, P., Piel, G., Dogné , J. M., Delattre, L., & Evrard, B. (2007). Solid lipid microparticles as a sustained release system for pulmonary drug delivery. European Journal ofPharmaceutics and Biopharmaceutics, 65, 47-56.

Jayaprakasha, G. K., Rao, L. J. M., & Sakariah, K. K. (2002). Improved HPLC method for the determination of curcumin, demethoxycurcumin and bisdemethoxycurcumin. Journal ofAgricultural and Food Chemistry, 50, 3668-3672.

Khopde, S., Priyadarsini, K. I., Venkatesan, P., & Rao, M. N. A. (1999). Free radical scavenging ability and antioxidant efficiency ofcurcumin and its substituted analogue. Biophysical Chemistry, 80, 83-89.

Kim, B. J., Kim, J. H., Kim, H. P., & Heo, M. Y. (1997). Biological screening of 100 plant extracts for cosmetic use (II): Anti-oxidative activity and free radical scavenging activity. International Journal of Cosmetic Science, 19, 299-307.

Lantz, R. C., Chen, G. J., Solyom, A. M., Jolad, S. D., & Timmermann, B. N. (2005). The effect of turmeric extracts on inflammatory mediator production. Phytomedicine, 12, 445-452.

Martin, A. (1993). Physical pharmacy (4 ed.). Philadelphia: Lea & Febiger.

Mehnert, W., & Mäder, K. (2001). Solid lipid nanoparticles: Production, characterization and applications. Aclvanced Drug Delivery Reviens, 47, 165-196.

Pfeiffer, E., Hohle, S., Solyom, A. M., & Metzler, M. (2003). Studies on the stability ofturmeric constituents. Journal ofFood Engineering, 56, 257-259.

Price, L. C., & Buescher, R. W. (1996). Decomposition of turmeric curcuminoids as affected by light, solvent and oxygen. Journal ofFoodBiochemistry,20, 125-133.

Sreejayan, N., & Rao, M. N. (1994). Curcuminoids as potent inhibitors of lipid peroxidation.

Journal ofPharmacy and Pharmacology, 46, 1013-1016.

Thakker, K. D., & Chern, W. H. (2003). Development and validation of in vitro release tests for semisolid dosage forms-case study. Dissolution Technologies, 10, 10-16.

Tiyaboonchai, W., Tungpradit, W., & Plianbangchang, P. (2007). Formulation and chamcterization of curcuminoids loaded solid lipid nanoparticles. International Journal of Pharmaceutics, 337, 299-306.

Tonnesen, H. H. (2002). Solubility, chemical and photochemical stability of curcumin in surfactant solutions. studies of curcumin and curcuminoids, XXVIII. Pharmazie, 57, 820-824.

Tonnesen, H. H., Karlsen, J., & van Henegouwen, G. B. (1986). Studies on curcumin and curcuminoids. VIII. photochemical stability of curcumin. Zeitschrift für

Lebensmitteluntersuchung und -Forschung A, l83, 116-122.

Trotta, M., Cavalli, R., Carlotti, M. E., Battaglia, L., Debernardi, F. (2005). Solid lipid micro-particles carrying insulin formed by solvent-in-water emulsion-diffusion technique. International Journal of Pharmaceutics, 288, 281-288.

Wang, Y. J., Pan, M. H., Cheng, A. L., Lin, L. I., Ho, Y. S., Hsieh, C. Y. et al. (1997). Stability of curcumin in buffer solution and characterization of its degradation products. Journal of Pharmaceutical and Biomedical Analysis, l5, 1867-1876.