เปรียบเทียบการยับยั้งเชื้อแบคทีเรียของสารสกัดจากเปลือกมังคุด ขมิ้นชันและใบบัวบก

Comparison of antimicrobial activity of Mangosteen crude, Turmeric and Gotu Kola extract

Abstract

This research work is a study of an antimicrobial activity test of 3 herbal plants extract, namely Mangosteen crude, Turmeric and Gotu Kola, on Propionibacterium acnes and Staphylococcus aureus. These 3 herbal plants were extracted and analysed for the amount of important chemical specie by HPLC technique. The result showed that 1.1929 mg of xanthone/g of Mangosteen crude, 0.8753 mg of curcumine/g of Turmeric, 0.0142 mg of asiaticoside/g of Gotu Kola and 0.0960 mg of asiatic acid/g of Gotu Kola were extracted. The antimicrobial activity test was performed for each extracted chemical specie. It was found that, at the same extracting concentration, Mangosteen crude extract induced wider inhibition zone than the Turmeric and Gotu Kola extract. The Minimal inhibitory concentration (MIC) of Mangosteen crude extract, Turmeric and Gotu Kola
extract to inhibit Propionibacterium acnes were 12.5, 25 and 200 mg/ml respectively and Staphylococcus aureus were 6.25,
12.5 and 200 mg/ml respectively. The Minimal bactericidal concentration (MBC) of Mangosteen crude extract, Turmeric
extract and Gotu Kola extract to disinfect Propionibacterium acnes were 25, 50 and 200 mg/ml respectively and Staphylococcus
aureus were 12.5, 25 and 200 mg/ml respectively. When comparing the effect of the 3 herbal plants extract on antimicrobial
activity, it was shown that the Mangosteen crude extract could inhibit Propionibacterium acnes and Staphylococcus aureus with
the lowest concentration (25 mg/ml) among the three herbal plants extract.