Effect of Methylobacterium radiotolerans ED5-9 with Capability of Producing Indole-3-Acetic Acid (IAA) and 1-Aminocyclopropane-1-Carboxylic Acid Deaminase on the Growth and Development of Murdannia loriformis (Hassk.) Rolla Rao & Kammathy under In Vitro Condition

Thanawut Prombunchachai; Nareeluk  Nakaew; Apichat  Chidburee; Siripun Sarin

Authors

Thanawut Prombunchachai Deparment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand 65000
Nareeluk Nakaew Deparment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand 65000
Apichat Chidburee Agricultural Technology Research Institute, Rajamangala University of Technology Lanna, Lampang, Thailand 52000
Siripun Sarin Deparment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand 65000

Abstract

This study aimed to evaluate the production of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase enzyme from endophytic Methylobacterium radiotolerans ED5-9, and to investigate the effects of M. radiotolerans ED5-9 on Murdannia loriformis, under tissue culture condition. M. radiotolerans ED5-9 was isolated from leaves of M. loriformis and cultured in vitro. It produced indole-3-acetic acid (IAA) with a concentration of 3.36 ± 0.20 µg/ml after incubation for 60 hr. Activity of ACC deaminase enzyme was observed at 365.05 ± 90.51 nmol of a-ketobutyrate/mg of protein/h. To test the effects of inoculation, the experiment was carried on by immersion of the explants of M. loriformis into M. radiotolerans ED5-9 suspension for 1, 3 and 5 min, and subsequently cultured on the MS medium in which 2 mg/l of IAA substance was used as the control. The results showed that duration time of the immersed explants in M. radiotolerans ED5-9 suspension for 5 min had the highest average number of shoots (6.00 ± 1.00 shoots per explants), root length (0.71 ± 0.26 cm) and dry weight (46.00 ± 5.40 mg). However, the above results of growth were similar to the growth of M. loriformis explants in the control. The results indicated the possible utilizing of M. radiotolerans ED5-9 can produce ACC deaminase enzyme and IAA to enhance growth and development of the M. loriformis explants under the in vitro condition.

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