Effect of Naringin on Insulin Resistance and Oxidative Stress in Fructose Fed Rats

Wachirawadee Malakul; Sirinat Pengnet

Authors

Wachirawadee Malakul Faculty of Medical Science, Naresuan University
Sirinat Pengnet Faculty of Medical Science, Naresuan University

Keywords:

Naringin, Fructose, Insulin resistance, Oxidative stress

Abstract

Consumption of fructose is associated with the development of insulin resistance. Naringin, the major grapefruit flavonoid, has antioxidant, lipid lowering, insulin sensitizing, and cardiovascular protective activities. Therefore, the aim of this study was to evaluate the effect of naringin treatment on insulin resistance and oxidative stress in fructose fed rats. Male rats were divided into three groups: control (C), fructose (F), and fructose+ naringin (FN). Fructose fed rats received 10% fructose (w/v) in the drinking water for 12 weeks. Naringin (100 mg/kg/day) was orally administered for the final 4 weeks of the study. At the end of the study, the levels of insulin and blood glucose, as well as an insulin resistance index (HOMA-IR) were determined. Hepatic and serum levels of malondialdehyde (MDA) and antioxidant enzyme, including superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were measured. Results showed that fructose-fed rats exhibited a significant increase in serum insulin, glucose, and HOMA-IR index. Fructose feeding also increased hepatic and serum MDA levels. Treatment of the fructose-fed rats with nargin reversed these alterations. These results suggest that naringin treatment for 4 weeks improves high fructose induced insulin resistance and oxidative stress in rats.

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