Allelopathic Effect of Barleria lupulina Lindl.on Germination and Seedling Growth of Pigweed and Barnyardgrass

Thanatsan Poonpaiboonpipat

Authors

Thanatsan Poonpaiboonpipat Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand

Keywords:

Allelopathy, Barleria lupulina Lindl, Inhibitory activity

Abstract

This study aimed to evaluate on an allelopathic potential and its chemical basis of Barleria lupulina Lindl., a traditional medicinal plant in Thailand. Pigweed (Amaranthus spinosus L.) and barnyard grass (Echinochloa crus-galli (L.) Beauv.) were used as test species. Aqueous extract of different parts at concentrations of 25, 50, 75 and 100 mg/mL was assayed on a seed germination and seedling growth of the test species. The leaf extract significantly showed the highest inhibitory activity of germination and seedling growth of the test species, followed by stems and roots respectively. The leaf selected on partially isolation of active compounds by acid-base partitioning method. Crude 70% aqueous-ethanol extract of leaf was further separated into acidic fraction (AE), neutral fraction (NE) and aqueous fraction (AQ). The fractions were assayed on the test species at the concentrations of 2,500, 5,000, 7,500 and 10,000 ppm. The inhibitory activity was significance depending on fractions and concentrations. NE fraction showed the most inhibition on germination and seedling growth of pigweed and barnyard grass followed by AE and AQ respectively. The inhibitory effect increased with increasing concentration. Pigweed were more susceptible to the fractions than barnyard grass. These results indicated that B. lupilina contained growth inhibitory substances and possess allelopathic activity. Then, partially isolation of active compounds showed NE fraction had the most effect. The results lead for further identification of allelochemicals and development into natural herbicides for sustainable agriculture.

References

Batish, D. R., Setia, N., Singh, H. P., & Kohli, R. K. (2004). Phytotoxicity of lemon-scented eucalypt oil and its potential use as a bioherbicide. Crop Protection, 23(12), 1209-1214.

Einhellig, F. A. (1986) Mechanisms and modes of action. In A. R. Putnam & C. S. Tang (Eds.). The science of allelopathy (pp. 171–188). New York: Wiley.

Inderjit, Streibig, J. C., & Olofsdotter, M. (2002). Joint action of phenolic acid mixtures and its significance in allelopathy research. Physiologia Plantarum, 114(3), 422-428.

Kanchanapoom, T., Kasai, R., & Yamasaki, K. (2001). Iridoid glucosides from Barleria lupulina. Phytochemistry 58(2), 337-341.

Khanh, T. D., Xuan, T. D., & Chung, I. M. (2007). Rice allelopathy and the possibility for weed management. Annals of Applied Biology, 151(3), 325-339.

Laosinwattana, C., Phuwiwat, W., & Charoenying, P. (2007). Assessment of allelopathic potential of Vetivergrass (Vetiveria spp.) ecotypes. Allelopathy Journal, 19(2), 469-478.

Laosinwattana, C., Poonpaiboonpipat, T., Teerarak, M., Phuwiwat, W., Mongkolaussavaratana, T., & Charoenying, P. (2009). Allelopathic potential of Chinese rice flower (Aglaia odorata Lour.) as organic herbicide. Allelopathy Journal, 24(1), 45-54.

Singh, H. P., Batish, D. R., Pandher, J. K., & Kohli, R. K. (2003). Assessment of allelopathic properties of Parthenium hysterophorus residues. Agriculture, Ecosystems & Environment, 95(2), 537-541.

Sisodia, S., & Siddiqui, M. B. (2010). Allelopathic effect by aqueous extracts of different parts of Croton bonplandianum Baill. on some crop and weed plants. Journal of Agricultural Extension and Rural Development, 2(1), 22-28.

Sodaeizadeh, H., Rafieiolhossaini, M., & Van Damme, P. (2010). Herbicidal activity of a medicinal plant, Peganum harmala L., and decomposition dynamics of its phytotoxins in the soil. Industrial Crops and Products, 31(2), 385-394.

Suksamrarn, A. (1986). Iridoid glucosides from Barleria lupulina. Journal of Natural Products, 49(1), 179-179.

Teerarak, M., Laosinwattana, C., & Charoenying, P. (2010). Evaluation of allelopathic, decomposition and cytogenetic activities of Jasminum officinale L. f. var. grandiflorum (L.) Kob. on bioassay plants. Bioresource Technology, 101(14), 5677-5684.

Tuntiwachwuttikul, P., Pancharoen, O., & Taylor, W. C. (1998). Iridoid glucosides of Barleria lupulina. Phytochemistry, 49(1), 163-166.

Uddin, M. R., Li, X., Won, O. J., Park, S. U., & Pyon, J. Y. (2012). Herbicidal activity of phenolic compounds from hairy root cultures of Fagopyrum tataricum. Weed Research, 52(1), 25-33.

Weston, L. A. (1996). Utilization of allelopathy for weed management in agroecosystems. Agronomy Journal, 88(6), 860-866.

Xuan, T. D., Shinkichi, T., Hong, N. H., Khanh, T. D., & Min, C. I. (2004). Assessment of phytotoxic action of Ageratum conyzoides L. (billy goat weed) on weeds. Crop protection, 23(10), 915-922.