Effect of Fermentation Broth from Indole-3-Acetic Acid (IAA) Producing Methylobacterium radiotolerans ED5-9 on the Growth and Development of Murdannia loriformis (Hassk.)  Rolla Rao & Kammathy under In vitro Condition

Thanawut Prombunchachai; Nareeluk Nakaew; Apichat Chidburee; Siripun Sarin

doi:10.14456/nujst.2019.11

Authors

Thanawut Prombunchachai Deparment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand 65000
Nareeluk Nakaew Deparment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand 65000
Apichat Chidburee Agricultural Technology Research Institute, Rajamangala University of Technology Lanna, Lampang, Thailand 52000
Siripun Sarin Deparment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand 65000

DOI:

https://doi.org/10.14456/nujst.2019.11

Keywords:

indole-3-acetic acid (IAA), Methylobacterium radiotolerans ED5-9, Murdannia loriformis, Tissue culture

Abstract

The possible role of indole-3-acetic acid (IAA) produced from Methylobacterium radiotolerans ED5-9 has been studied in the tissue culture of Murdannia loriformis for 4 weeks. The concentration of IAA in liquid nutrient medium (LNM) of M. radiotolerans ED5-9 culture was 3.36 ± 0.20 µg/ml. This supernatant was mixed with Murashige and skoog (MS) medium in varying volumes (1, 2 and 4 ml/l) and compared with MS medium supplemented with IAA for the tissue culture of M. loriformis. Results show that bacterial IAA can promote plant growth in terms of increasing number of shoots, roots and leaves as well increases in root length and dry weight more than chemically synthesized IAA. Root formation and increase in root length was found to be highest in treatments having 2 ml/l bacterial supernatant in 0.8% agar medium with an average of 11.00 ± 4.60 roots per explants and 2.10 ± 0.70 cm, respectively. In addition, Total phenolic compounds and antioxidant activity were found in quantity as 0.36 ± 0.46 and 4.14 ± 0.52 mg/g fresh weight, respectively. As the concentration of IAA in supernatant from M. radiotolerans ED5-9 was increased, the number of roots also increased.

References

Ali, B., & Hasnain, S. (2007) Efficacy of bacteria auxin on in vitro growth of Brassica oleracea L. World Journal of Microbiology and Biotechnology, 23(6), 779-784.

Ali, B., & Hasnain, S. (2007) Potential of bacteria indoleacetic acid to induce adventitious shoots in plant tissue culture. Letters in Applied Microbiology, 45(2), 128-133.

Andrews, M. Sprent, J. I., Raven, J. A., & Eady, P. E. (1999) Relationships between shoot and root ratio, growth and leaf soluble protein concentration of Pisum sativum, Phaseolus vulgaris and Triticum aestivum under different nutrient deficiencies, Pant, Cell and Environmental, 22(8), 949-958.

Arroo, R. R. J., Develi, A., Meijers, H., Van de Westerlo, E., Kemp, A. K., Croes, A. F., & Wullems, G. J. (1995) Effect of exogenous auxin on root morphology and secondary metabolism in Tagetes patula hairy root cultures. Physiologia Plantarum, 93(2), 233-240.

Davies, P. J. (2004) The Plant Hormones: Their Nature, Occurrence, and Function. In P. J. Davies (Ed.), Plant Hormones: Biosynthesis, Signal Transduction, Action (pp.1-15). Netherlands: Kluwer Academic Publishers.

Debnath, M., Malik, C. P., & Bisen, P. S. (2006) Micropropagation: a tool for the production of high quality plant-based medicines. Current Pharmaceutical Biotechnology, 7(1), 33-49.

Devi, R. P., Sundaram, S. P., & Poorniammal, R. (2010) Effect of facultative methyltrophs on tissue culturing of rice. Asian Journal of Bio Science, 4(2), 207-209.

Doronina, N. V., Ivanova, E. G., & Trotsenko, Yu. A. (2002) New evidence for the ability of methylobacteria and methanotrophs to synthesize auxins. Microbiology, 71(1), 130-132.

Glickmann, E., & Dessaux, Y. (1995) A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Applied and Environmental Microbiology, 61(2), 793-796.

Green, P. N. (2006) Methylobacterium. In M. Dworkin, S. Falkow, E. Rosenberg, K. Schleifer, & E. Stackebrandt (Eds.), The Prokaryotes Vol.5 (pp. 257-265). Singapore: Springer Science+Business Media, LLC.

Hsh, C. Y. (2006) Antioxidant activity of extract from Polygonum aviculare L. Biological Research, 39(2), 281-288.

Ivanova, E. G., Doronina, N. V., & Trotsenko, Yu. A. (2001) Aerobic methylobacteria are capable of synthesizing auxins. Microbiology, 70(4), 452-458.

Jiratchariyakul, W., Okabe, H., Moongkarndi, P., & Frahm, A. W. (1998) Cytotoxic glycosphingolipid from Murdannia loriformis (Hassk.) Rolla Rao & Kam. Thai Journal of Phytopharmacy, 5(1), 10-20.

Jiratchariyakul, W., Vongsakul , M., Sunthornsuk, L., Moongkarndi, P., Narintorn, A., Somanabandhu, A., … & Frahm, A. W. (2006) Immunomodulatory effect and quantitation of cytotoxic glycosphingolipid from Murdannia loriformis. Journal of Natural Medicines, 60(3), 210-216.

Kalyaeva, M. A., Ivanova, E. G., Doronina, N. V., Zakharchenko, N. S., Trotsenko, Yu. A., & Buryanov, Ya. I. (2003) The Effect of aerobic methylotrophic bacteria on the In vitro morphogenesis of soft wheat (Triticum aestivum). Russian Journal of Plant Physiology, 50(3), 313-317.

Kousalya, L., & Bai, V. N. (2016). Effect of growth regulators on rapid micropropagation and antioxidant activity of Canscora decussata (Roxb.) Roem. & Schult. – A threatened medicinal plant. Asian Pacific Journal of Reproduction, 5(2), 161-170.

Kutschera, U. (2007) Plant-associated methylobacteria as Co-evolved phytosymbionts. Plant Signaling and Behavior, 2(2), 74-78.

Lichtenthaler, H. K., & Buschmann, C. (2001) Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy. In R. E. Wrolstad, T. E. Acree, E. A. Decker, M. H. Penner, D. S. Reid, S. J. Schwartz, C. F. Shoemaker, D. M. Smith & P. Sporns (Eds.), Current Protocols in Food Analytical Chemistry (pp. F4.3.1-F4.3.8). N.P.: John Wiley and Sons.