Effects of Different Nitrogen Forms on Growth, Phenolic Content, and Antioxidant Activity in Hedychium speciosum and H. coronarium

Janjira Muenrew; Ratchuporn Suksathan; Ratchadawan Puangpradab; Apinya Rachkeeree; Kuttiga Kantadoung

doi:10.14456/nujst.2021.14

Authors

Janjira Muenrew Queen Sirikit Botanic Garden, P.O. Box 7, Mae Rim, Chiang Mai 50180, Thailand
Ratchuporn Suksathan Queen Sirikit Botanic Garden, P.O. Box 7, Mae Rim, Chiang Mai 50180, Thailand
Ratchadawan Puangpradab Queen Sirikit Botanic Garden, P.O. Box 7, Mae Rim, Chiang Mai 50180, Thailand
Apinya Rachkeeree Queen Sirikit Botanic Garden, P.O. Box 7, Mae Rim, Chiang Mai 50180, Thailand
Kuttiga Kantadoung Queen Sirikit Botanic Garden, P.O. Box 7, Mae Rim, Chiang Mai 50180, Thailand

DOI:

https://doi.org/10.14456/nujst.2021.14

Keywords:

Phenolic content, Antioxidant activity, Nitrogen forms, Hedychium species, Growth, Morphology

Abstract

Nitrogen (N) is the most important nutrient element for plant growth and the synthesis of many secondary metabolites. Plants typically obtain inorganic N in form of NH₄⁺ and NO₃^-. Two Hedychium species were grown in nutrient solutions modified from Smart and Barko (1985). The experiment consists of three N forms; NH₄⁺, NH₄⁺NO₃^-, NO₃^- and control (no N form) with the same concentration of nitrogen (500 µM) for 60 days. Destructive sampling was done and morphology was recorded. Total phenolic content (TPC) was evaluated by Folin-Ciocalteu method while the antioxidant activity was evaluated by DPPH and ABTS radical scavenging activity. Different nitrogen forms have significantly (p ≤0.05) affected plant height, root numbers, root number and total biomass between two Hedychium species. H. speciosum had height new shoot, leaf number, root number and total biomass in NH₄⁺ supply. H. coronarium had height, leaf number, root number and total biomass in NH₄⁺NO₃^- supply. Total phenolic contents both species were increased by NH₄⁺ supply. H. speciosum were accumulate total phenolic content in the leaves 21.55 mg.g^-1 GAE was lower than in H. coronarium 43.49 mg.g^-1GAE, but in stem of the H. speciosum were accumulate total phenolic content 25.08 mg.g^-1GAE was lower than in H. coronarium 20.66 mg.g^-1GAE. NH₄⁺ supply increased antioxidant activity with DPPH radical scavenging activity and ABTS radical scavenging activity.

References

Brand-Williams, W., Cuvelier, M. E., & Berset, C. L. W.T. (1995). Use of a free radical method to evaluate Antioxidant activity. LWT - Food Science and Technology, 28(1), 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5

Britto, D. T., & Kronzucker H. J. (2002). NH4+ toxicity in higher plants: A critical re-view. Journal of Plant Physiology, 159, 567-584. http://dx.doi.org/10.1078/0176-1617-0774

Caldwell, M. M., Ballare, C. L., Bornman, J. F., Flint, S. D., Bjorn, L. O., Teramura, A. H. … Tevini, M. (2003). Terrestrial ecosystems, increased solar ultraviolet radiation and interactions with other climatic change factors. Photochemical & Photobiological Sciences, 2, 29–38.

Cartea, M. E., Francisco, M., Soengas, P., & Velasco, P. (2011). Phenolic Compounds in Brassica Vegetables. Journal Molecular, 16, 251-280; https://doi.org/10.3390/molecules16010251

Esmaeili, A., Tavassoli, A., & Ebrahimzadeh, M. A. (2009). Antioxidant activity and free radical scavenging activity of Salvia glutinosa growing in Iran. Pharmacology online, 2, 109–116.

Gweyi-Onyango, J. P., Neumann, G., & Roemheld, V. (2009). Effects of Different Forms of Nitrogen on Relative Growth Rate and Growth Components of Tomato (Lycopersicon esculentum Mill.). African Journal of Horticultural Science, 2, 43-55.

Hartatia, R., Sugandaa, A.G., & Fidriannya, I. (2014). Botanical, Phytochemical and Pharmacological Properties of Hedychium (Zingiberaceae) - A Review. Procedia Chemistry, 13, 150-163.

Horchani, F., Aloui, A., Brouquisse, R., & Aschi-Smiti, S. (2010). Physiological responses of tomato plants (Solanum lycopersicum) as affected by root hypoxia and NaCl salinity. Agronomy and Crop Science, 194, 297-303. https://doi.org/10.1111/j.1439-037X.2008.00313.x

Ibrahim, M. H., Jaafar, H. Z., Rahmat, A., & Rahman, Z. A. (2011). Effects of nitrogen fertilization on synthesis of primary and secondary metabolites in three varieties of Kacip Fatimah (Labisia pumila Blume). International Journal of Molecular Sciences, 12, 5238-5254. https://doi.org/10.3390/ijms

Jain, S. P., Singh, J., & Singh, S. C. (2003). Rare endangered medicinal and aromatic plants of Madhya Pradesh. Journal of economic and taxonomic botany, 27(4), 925-932.

Kova, C. J., Klejdus, B., Backor, M., & Rep, C. M. (2007). Phenylalanine ammonia-lyase activity and phenolic compounds accumulation in nitrogen-deficient Matricaria chamomilla leaf rosettes. Journal of Plant Sciences, 172(2), 393-399. https://doi.org/10.1016/j.plantsci.2006.10.001

Minu, S. M., Masroor, A., & Khan, M. N. (2016). Effect of nitrogen on growth, nutrient assimilation, essential oil content, yield and quality attributes in Zingiber officinale Rosc. Journal of the Saudi Society of Agricultural Sciences, 15(2), 171-178. https://doi.org/10.1016/j.jssas.2014.11.002

Munene, R., Changamu, E., Korir, N., Onyango, & Joseph, G. (2017). Effects of different nitrogen forms on growth, phenolics, flavonoids and antioxidant activity in amaranth species. Tropical plant research, 4(1), 81–89. https://doi.org/10.22271/tpr.2017.v4.i1.012

Nabavi, S. M., Ebrahimzadeh, M. A., Nabavi, S. F., Hamidinia, A., & Bekhradnia, A. (2008). Determination of antioxidant activity, phenol and flavonoids content of Parrotia persica Mey. Pharmacology online, 2, 560-567.

Pachurekar, P., & Dixit, A. K. (2017). A Review on Pharmacognostical Phytochemical and Ethnomedicinal Properties of Hedychium Coronarium J. Koenig an Endangered Medicine. International Journal of Chinese Medicine, 1(2), 49-61. http://dx.doi.org/10.11648/j.ijcm.20170102.13

Razaq, M., Zhang, P., Shen, H., & Salahuddin. (2017). Influence of nitrogen and phosphorus on the growth and root morphology of Acer mono. PLoS One, 12, 1–13. https://doi.org/10.1371/journal.pone.0171321

Sabir, M., Hanafi, M. M., Malik, M. T., Aziz, T., Zia-ur-Rehman, M., Ahmad H. R., … Shahid, M. (2013) Differential effect of nitrogen forms on physiological parameters and micronutrient concentration in maize (Zea mays L.). Australian Journal of Crop Science-AJCS, 7(12), 1836-1842.

Salahas, G., Papasavvas, A., Giannakopoulos, E., Tselios, T., & Savvas, D. (2011). Impact of nitrogen deficiency on biomass production, gas exchange, and betacyanin and total phenol concentrations in red beet (Beta vulgaris L.) Plants. European Journal of Horticultural Science, 76(5), 194-200.

Saxena, M., Saxena, J., Nema, R., Singh, D., & Gupta, A. (2013). Phytochemistry of medicinal plants. J. Pharm. Phytochem, 1(6), 168-82.

Schlesier, K., Harwat, M., Böhm, V., & Bitsch, R. (2002). Assessment of Antioxidant Activity by Using Different in Vitro Methods. Free Radical Research, 36(2), 177-87.

Suksathan, R., Puangpradab, R., Saratan, N., & Boonvun, D. (2018). Bioactive compounds and antioxidant properties of four Hedychium flowers for flavoured tea. Acta Horticulturae, 1194, 1053-1056. https://doi.org/10.17660/ActaHortic.2018.1194.150

Smart, R. M., & Barko, J. W. (1985). Laboratory culture of submersed freshwater macrophytes on natural sediments. Aquatic Botany, 21, 251-263. https://doi.org/10.1016/0304-3770(85)90053-1

Tischner, R. (2000). Nitrate uptake and reduction in higher and lower plants. Plant Cell Environment, 23, 1055-1024. https://doi.org/10.1046/j.1365-3040.2000.00595.x