Isolation and Characterization of Bacteriophage which Infected  Klebsiella pneumoniae

Sunsiree Muangman; Jiraporn Gatedee

doi:10.14456/nujst.2022.39

Authors

Sunsiree Muangman Department of Medical Sciences, Faculty of Science, Rangsit University, Pathum Thani, 12000, Thailand
Jiraporn Gatedee Faculty of Medical Technology, Rangsit University, Pathum thani, Thailand 12000

DOI:

https://doi.org/10.14456/nujst.2022.39

Keywords:

K, pneumoniae, bacteriophages, biofilm, Myoviridae

Abstract

Klebsiella pneumoniae is a clinically significant organism that has caused much public health concern. Due to the increasing prevalence of infections caused by multidrug-resistant K. pneumoniae, alternative therapies might be promising. The properties of host specificity, abundance in nature, and evolvability suggest bacteriophages might be a good candidate. In this study, we have isolated and characterized a lytic bacteriophage infecting K. pneumoniae, ØKN-2, which was isolated from wastewater. The transmission electron micrograph showed that ØKN-2 was classified as being in the Myoviridae family. Host-range determination revealed that ØKN-2 is specifically able to lyse 35.7% (5/14) of tested K. pneumoniae. Studies of the host range of different species showed that ØKN-2 unable to lyse other tested bacteria including Acinetobacter baumannii, Peudomonas aeruginosa, Protues mirabilis, Enterococcus faecalis, and Escherichia coli. Interestingly, ØKN-2 significantly reduced K. pneumoniae ATCC 700603 biofilm preformed in a dose dependent manner. Therefore, this study identified and tested bacteriophages that infect K. pneumoniae and proved the effectiveness of ØKN-2 against biofilm and host-specific characteristics which indicates that they are beneficial in the development of treatments against K. pneumoniae infections.

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