Phytotoxicity Activities of Essential Oil of Pinus sylvestris against Zea mays, Solanum lycopersicum, and Vigna unguiculata as Potential Bio-pesticides in Africa

Shola  Hezekiah Awojide; Grace  Temitayo Ajayi; Kehinde  Adenike Oyewole; Adeniyi  Solomon Tayo; Adebayo  Emmanuel Adeleke; Ezekiel  Olumide Fadunmade

doi:10.14456/nujst.2023.34

Authors

Shola Hezekiah Awojide Department of Pure and Applied Chemistry, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, 210001, Nigeria
Grace Temitayo Ajayi Department of Pure and Applied Chemistry, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, 210001, Nigeria
Kehinde Adenike Oyewole Department of Chemical Engineering, Faculty of Engineering, Osun State University, Osogbo, 210001, Nigeria
Adeniyi Solomon Tayo Department of Pure and Applied Chemistry, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, 210001, Nigeria
Adebayo Emmanuel Adeleke Department of Basic Sciences, Faculty of Science, Adeleke University, Ede, 232104, Osun State, Nigeria
Ezekiel Olumide Fadunmade Department of Pure and Applied Chemistry, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, 210001, Nigeria

DOI:

https://doi.org/10.14456/nujst.2023.34

Keywords:

Pinus sylvestris, Phytotoxicity, Herbicide, Essential oil

Abstract

The chemical composition of the essential oil of Pinus sylvestris (Scots pine), as well as its phytotoxicity action against Zea mays, Vigna Unguiculata, and Solanum lycopersicum, were investigated in this study. The essential oil from twigs of P. sylvestris was extracted using the hydro-distillation method. The major component of the essential oil was characterised by GC/MS, and the inhibitory phytotoxicity of the essential oil formulation at varying concentrations (1, 2, 3, 4, 5 mL/L) was determined by measuring the length of the shoots and the roots of tested plants. The phytotoxicity effect of the essential oil formulations on the three seeds was also determined on the foliar as well as the root site of the three seedlings by recording the number(s) of seedlings which were distressed. The major chemical constituents are mainly oxygenated monoterpenes. The compounds are Longifolene (5.45%), Borneol (6.72%), β -terpineol (14.07%), Terpinen-4-ol (21.82%) and α-terpineol (27.17%). The root and shoot inhibitory toxicity indicated that phytotoxicity was highest on the seeds of V. unguiculata. A varying degree of toxicity was reported on the activity of the essential oil on the leaves of the seedlings of Z. mays, S. lycopersicum and V. unguiculata after 24 hr. S. lycopersicum got the lowest level of toxicity when tested on the roots and leaves of seedlings. The seedlings of Z. mays and V. unguiculata showed 100% distress when 4 mL/L of the essential oil formulation was applied, while S. lycopersicum recorded 30% distress of seedlings. All phytotoxicity activities observed were time and dose-dependent. The essential oil of P. sylvestris showed appreciable phytotoxicity activity against the three tested plants; hence, it may be considered to be a potential bioherbicide.

References

Angelini, L.G., Carpanese, G., Cioni, P.L., Morelli, I., Macchia, M., & Flamini, G., (2003). Essential oils from Mediterranean Lamiaceae as weed germination inhibitors. Journal of Agricultural and Food Chemistry, 51, 6158-6164.Retrieved from https://www.jnsciences.org/index.php?option=com_attachments&task=download&id=207

Awojide, S. H, Oyewole, K. A., Abiona, O. O., & Agbaje, A. W. (2021). Phytotoxicity of essential oil from Piper nigrum L. on some selected food crops as a potential herbicide in Africa. African Journal of Pure and Applied Science, 2(2), 93–99. https://doi.org/10.33886/ajpas.v2i2.212

Awojide, H. S., Adegbite, A. O., Oyewole, A. K., Adeyemo, G. A., Fadunmade, E. O., & Anifowose, A. J. (2023). The effect of fractionation of the essential oil from Pinus sylvestris on the synergistic and antagonistic medicinal activities. Kabale University Interdisciplinary Research Journal, 2(1), 141 – 154.

Babushok, V. I., Linstrom, P. J., & Zenkevich, I. G. (2011). Retention Indices for frequently reported compounds of plant essential oils. Journal of Physical and Chemical Reference Data, 40(4), 043101-043146. https://doi.org/10.1063/1.3653552

Blázquez, M. A., & Ibáñez, M. D. (2020). Phytotoxic effect of commercial essential oils on selected vegetable crops: cucumber and tomato. Sustainable Chemistry and Pharmacy, 15, 100209. https://doi.org/10.1016/j.scp.2019.100209

De Martino L., Formisano C., Mancini E., De Feo V., Piozzi F., Rigano D., Senatore F., (2010). Chemical composition and phytotoxicity effects of essential oils from four Teucrium species. Natural Product Communication, 5, 1969-1976. https://doi.org/10.1177/1934578X1000501230

Dhifi, W., Bellili, S., Jazi, S., Bahloul, N., & Mnif, W. (2016). Essential oils’ chemical characterization and investigation of some biological activities: A critical review. Medicines, 3, 25. https://doi.org/ 10.3390/medicines3040025

Gharde, Y., Singh, P. K., Dubey, R. P., & Gupta, P. K. (2018). Assessment of yield and economic losses in agriculture due to weeds in India. Crop Protection, 107, 12-18. https://doi.org/10.1016/j.cropro.2018.01.007

Grichi A., Nasr Z., Khouja M. L (2016). Phytotoxic effects of essential oil from Eucalyptus cinerea and its physiological mechanisms. Journal of New Sciences, Agriculture and Biotechnology. 13:1289–1296.

Ibáñez, M. D., & Blázquez, M. A. (2019). Phytotoxic effects of commercial Eucalyptus citriodora, Lavandula angustifolia, and Pinus sylvestris essential oils on weeds, crops, and invasive Species. Molecules, 24, 2847. https://doi.org/10.3390/molecules24152847

Ismail, A., Lamia, H., Mohsen, H., & Bassem, J. (2013). Reviews on phytotoxic effects of essential oils and their individual components: New approach for weeds management. International Journal of Applied Biology and Pharmaceutical Technology, 4(1), 96-114.

Kordali, S., Cakir, A., & Sutay, S. (2007). Inhibitory effects of monoterpenes on seed germination and seed growth. Zeitschrift fur Naturforschung C, 62(34), 207-214. https://doi.org/10:1515/znc-2007-3-409

Kucharska, M., Szymańska, J. A, Wesołowski, W., Bruchajzer, E., & Frydrych, B. (2018). Comparison of chemical composition of selected essential oils used in respiratory diseases. Medycyna Pracy, 69(2), 167–178. https://doi.org/10.13075/mp.5893.00673

Kumar, P., & Dwivedi, P. (2018). “Cadmium-induced alteration in leaf length, leaf width and their ratio of glomus treated sorghum seed”. Journal of Pharmacognosy and Phytochemistry, 6, 138-141.

Kumar, P, Kumar, S., Naik, M., Yummam, J., & Purnima. (2018). “Glomus and putrescine based mitigation of cadmium-induced toxicity in maize”. Journal of Pharmacognosy and Phytochemistry, 7(5), 2384- 2386.

Kumar, P., Misao, L., Jyoti, N., Swaraj, K., Harshavardhan, M., Purnema, & Naik, M. (2018). “Polyamines and Mycorrhiza based mitigation of cadmium-induced toxicity for plant height and leaf number in maize”. International Journal of Chemical Studies, 6(5), 2491- 2494.

Matłok, N., Gorzelany, J., Piechowiak, T., & Balawejder, M. (2020). Influence of drying temperature on the content of bioactive compounds in scots pine (Pinus sylvestris L.). Shoots as well as yield and composition of essential oils. Acta Universitatis Cibiniensis Series E: Food Technology, 24(1), 15-24.

Mehdizadeh, L., & Moghaddam, M. (2018). Essential oils: Biological activity and therapeutic potential. In Therapeutic, Probiotic and Unconventional Foods, 167–176. https://doi.org/10.1016/B978-0-12-814625-5.00010-8

Mitić, Z. S., Jovanović, B., Jovanović, S., Mihajilov-Krstev, T., Stojanović-Radić, Z. Z., Cvetković, V. J., … Stojanović, G. S. (2018) Comparative study of the essential oils of four Pinus species: Chemical composition, antimicrobial and insect larvicidal activity. Industrial Crops Production, 111, 55–62. https://doi.org/10.1016/j.indcrop.2017.10.004

Morsy, N. F. S. (2017). Chemical structure, quality indices and bioactivity of essential oil constituents. In Active Ingredients from Aromatic and Medicinal Plants. InTech, 2017, 175–206. https://doi.org/10.5772/66231.

Rassaeifar, M., Hosseini, N., Asl, N. H. H., Zandi, P., & Aghdam, A. M. (2013). Allelopathic effect of eucalyptus globulus’ essential oil on seed germination and seedling establishment of Amaranthus blitoides and Cyndon dactylon. Trakia Journal of Sciences, 11(1), 73-81.

Sara, B., Mohamed, H., Nadjia, Z., & Abdelkrim, H. (2019). Chemical composition and herbicidal activity of essential oils from two Labiatae species from Algeria. Journal of Essential Oil Research, 2019, 1-12. https://doi.org/10.1080/10412905.2019.1567400

Uremis, M., Arslan, & Sangun, M. K. (2009). Herbicidal Activity of Essential Oils on the Germination of Some Problem Weeds. Asian Journal of Chemistry, 21(4), 3199-3210.

Vishwakarmaa, G. S., & Mittala, S. (2014). Bioherbicidal potential of essential oil from leaves of Eucalyptus tereticornis against Echinochloa crus - galli L. Journal of Biopest, 5(1), 47-53.