Comparison of Dwarf Water Hyssop (Bacopa monnieri) in Emerged and Submerged Systems in Aquariums

Pornpimon Chuaduangpui; Chanraksmey Tauk; Tassanee Khawniam

doi:10.69650/ahstr.2024.1008

Authors

Pornpimon Chuaduangpui Faculty of Natural Resources, Prince of Songkla University, Hat Yai, 90112, Thailand
Chanraksmey Tauk Faculty of Natural Resources, Prince of Songkla University, Hat Yai, 90112, Thailand
Tassanee Khawniam Faculty of Natural Resources, Prince of Songkla University, Hat Yai, 90112, Thailand

DOI:

https://doi.org/10.69650/ahstr.2024.1008

Keywords:

aquatic plant, aquaculture, NPK fertilizer formulas, growth performance, survival rate

Abstract

Dwarf water hyssop (Bacopa monnieri) is a popular choice among aquarium enthusiasts for its ease of care and rapid growth. Despite its popularity, research on its suitability as an aquarium plant is scarce. The objective of this study was to assess the effects of various fertilizer formulas and growth performance in both emerged and submerged culture systems. Dwarf water hyssop was cultured for four weeks in emerged and submerged systems using different NPK fertilizer formulas (18-18-18, 25-5-5, 30-20-10, and 8-24-24), alongside a control group without fertilizer. Remarkably, growth performance (i.e. plant height, number of leaves, number of nodes, and root length) and survival rate did not significantly differ between plants with and without fertilizer application in either culture system. Among the fertilizers tested, treatment 4 (NPK 30-20-10) showed the most promising results for dwarf water hyssop growth in both emerged and submerged culture systems. In the emerged system, these treatments led to a minimum height increase (7.96±1.49 cm), a medium number of new leaves (4.41±1.91 leaves/explant), a moderate number of new nodes (3.08±0.29 nodes/explant), and a substantial root system length (2.67±1.16 cm). Conversely, in the submerged system, a minimum height increase (1.65±0.32 cm), medium number of new leaves (1.33±1.66 leaves/explant), moderate number of new nodes (1.08±0.76 nodes/explant), and root system length (0.36±0.86 cm) were observed. Overall, plant morphology and growth performance were superior in the emerged system compared to the submerged system after four weeks of culture. Outstandingly, all plants survived, regardless of fertilizer application of formula, in both culture systems. Given its slow growth, dwarf water hyssop is ideal for aquarists seeking a dwarf aquatic decoration. The emerged culture system is recommended for mass production of this ornamental plant, while the submerged culture system is suitable for elaborate ornamental aquariums.

References

Aquatic Mag. (2019). Bacopa monnieri caresheet. https://aquaticmag.com/aquarium-plants/bacopa-monnieri-caresheet/

Banerjee, M., & Sharivastava, S. (2008). An improved protocol for in vitro multiplication of Bacopa monnieri (L.). World Journal of Microbiology and Biotechnology, 24, 1355-1359. https://doi.org/10.1007/s11274-007-9612-3

Carr, G. M., Duthie, H. C., & Taylor, W. D. (1997). Models of aquatic plant productivity: A review of the factors that influence growth. Aquatic Botany, 59, 195–215. https://doi.org/10.1016/S0304-3770(97)00071-5

Crang, R., Lyons-Sobaski, S., & Wise, R. (2018). Plant anatomy: A concept-based approach to the structure of seed plants. Springer Cham. https://doi.org/10.1007/978-3-319-77315-5

Fan, Y., Macho, G. M., Jobbágy, E. G., Jackson, R. B., & Casal, C. O. (2017). Hydrologic regulation of plant rooting depth. Proceedings of National Academy of Sciences, 114(40), 10572-10577. https://doi.org/10.1073/pnas.1712381114

Grossnickle, S. C. (2005). Importance of root growth in overcoming plant stress. New Forests, 30, 273-294. https://doi.org/10.1007/s11056-004-8303-2

Hiscock, P. (2003). Encyclopedia of aquarium plants. Barron’s Educational Series.

Jathina, M., & Abdussalam, A. K. (2019). Histological and morphological response of Bacopa monnieri (L.) Pennell in glycophytic and halophytic condition. International Journal of Pharmaceutical Science and Research, 10(1), 407-411. https://doi.org/10.13040/IJPSR.0975-8232.10(1).407-11

Karatas, M., & Aasim, M. (2014). Efficient in vitro regeneration of medicinal aquatic plant water hyssop (Bacopa monnieri L. Pennell). Pakistan Journal of Agricultural Sciences, 51(3), 667-672. http://www.pakjas.com.pk

Karatas, M., Aasim, M., Dogan, M., & Khawar, K. M. (2013). Adventitious shoot regeneration of the medicinal aquatic plant water hyssop (Bacopa monnieri L. Pennell) using different internodes. Archives of Biological Sciences, 65(1), 297-303. https://doi.org/10.2298/ABS1301297K

Khalid, K. A., & Shedeed, M. R. (2015). Effect of NPK and foliar nutrition on growth, yield and chemical constituent in Nigella sativa L. Journal of Materials and Environmental Science, 6(6), 1709-1714. http://www.researchgate.net

Kunakhonnuruk, B., Inthima, P., & Kongbangkerd, A. (2023). Improving bacoside yield of Bacopa monnieri (L.) Wettst. in temporary immersion system by increasing immersion time and lowering the intervals. Industrial Crops & Products, 191, 115859. https://doi.org/10.1016/j.indcrop.2022.115859

Laohavisuti, N., Ruangdej, U., & Wangwibulkit, S. (2018). Effect of Kinetin and IAA on growth and antioxidants in Bacopa monnieri. King Mongkut’s Agricultural Journal, 35(2), 76-83. https://li01.tci-thaijo.org/index.php/agritechjournal/article/download/158101/114505/432900

Lawson, J. W., Fennell, M., Smith, M. W., & Bacon, K. L. (2021). Regeneration and growth in crowns and rhizome fragments of Japanese knowtweed (Reynoutria japonica) and desiccation as a potential control strategy. PeerJ, 9, e11783. https://doi.org/10.7717/peerj.11783

Le, X. T., Pham, H. T. N., Nguyen, T. V., Nguyen, K. M., Tanaka, K., Fujiwara, H., & Matsumoto, K. (2015). Protective effects of Bacopa monnieri on ischemia-induced cognitive deficits in mice: The possible contribution of bacopaside I and underlying mechanism. Journal of Ethnopharmacology, 164, 37-45. https://doi.org/10.1016/j.jep.2015.01.041

Marschner, P. (2012). Marschner's mineral nutrition of higher plants (3rd ed.). Elsevier Academic Press. https://doi.org/10.1016/C2009-0-63043-9

Razaq, M., Zhang, P., Shen, H., & Salahuddin. (2017). Influence of nitrogen and phosphorous on the growth and root morphology of Acer mono. PLoS ONE, 12(2), e0171321. https://doi.org/10.1371/journal.pone.0171321

Saha, P. S., Sarkar, S., Jeyasri, R., Muthuramalingam, P., Ramesh, M., & Jha, S. (2020). In Vitro Propagation, Phytochemical and Neuropharmacological Profiles of Bacopa monnieri (L.) Wettst.: A Review. Plants, 9(4), 411. https://doi.org/10.3390/plants9040411

Samarakoon, U. C., Weerasinghe, P. A., & Weekrakkody, W. A. P. (2006). Effect of electrical conductivity [EC] of the nutrient solution on nutrient uptake, growth, and yield of leaf lettuce (Lactuca sativa L). in stationary culture. Tropical Agricultural Research, 18, 13-21. http://www.researchgate.net

Sandoval, J. R. (2018). Bacopa monnieri (water hyssop). https://www.cabidigitallibrary.org/doi/10.1079/cabicompendium.112638

Sanputawong, S., Raknim, T., & Khawniam, T. (2018). Effects of plant growth regulator and activated charcoal on callus formation and plant regeneration of Bacopa monnieri (L.). Songklanakarin Journal of Plant Science, 5(1), 7-12. http://www.sjplantscience.com

Sinha, S., Gupta, M., & Chandra, P. (1996). Bioaccumulation and biochemical effects of mercury in the plant Bacopa monnieri (L.). Environmental Toxicology and Water Quality, 11, 105-112. https://doi.org/10.1002/(SICI)1098-2256(1996)11:2<105: AID-TOX5>3.0.CO;2-D

Subashri, B., & Koilpillai, K. Y. (2014). In vitro regeneration of Bacopa monnieri (L.) Pennel: A multipurpose medicinal plant. International Journal of Pharmacy and Pharmaceutical Science, 6(4), 559-563. http://www.researchgate.net

Tauk, C., Chuaduangpui, P., & Khawniam, T. (2021). Effects of explants on plant regeneration and cincentration of paclobutrazol on morphological responses of Dwarf water hyssop (Bacopa monnieri). Narasuan University Journal: Science and Technology, 29(3), 56-66. https://doi.org/10.14456/nujst.2021.26

Tropica. (2019). Bacopa ‘Compact’. https://tropica.com/en/plants/plantdetails/Bacopa'Compact'(044A)/4467