Bael Leaf Oil Increases AQP3 Expression and Exerts Wound-Healing  Effect in Human Immortalized Keratinocytes

Budsarin Kesornnoi; Orawin Prangsaengtong; Duangratana Chuvisitkul; Worapan Sitthithaworn

doi:10.14456/nujst.2023.26

Authors

Budsarin Kesornnoi Department of Pharmacognosy, Faculty of Pharmacy, Srinakharinwirot University, Nakonnayok, 26120, Thailand
Orawin Prangsaengtong Department of Biopharmacy, Faculty of Pharmacy, Srinakharinwirot University, Nakonnayok, 26120, Thailand
Duangratana Chuvisitkul Department of Pharmaceutical Technology, Faculty of Pharmacy, Srinakharinwirot University, Nakonnayok, 26120, Thailand
Worapan Sitthithaworn Department of Pharmacognosy, Faculty of Pharmacy, Srinakharinwirot University, Nakonnayok, 26120, Thailand

DOI:

https://doi.org/10.14456/nujst.2023.26

Keywords:

bael leaf oil, trans-caryophyllene, HaCaT, wound healing, aquaporin-3

Abstract

Bael (Aegle marmelos L. Corrêa, Family Rutaceae) leaf oil has been reported to promote wound healing in keratinocyte cell models. The oil key components, according to GC-MS analysis, are trans-caryophyllene and limonene. In this study, we investigated the mechanism by which bael leaf oil and its key components aid wound healing by focusing on the water channel aquaporin-3 (AQP3), which is crucial for maintaining skin hydration and identifying the active component responsible for the activity. To this objective, keratinocyte (HaCaT) cells were grown in media containing 2% FBS and subjected to a wound healing assay. The expression of AQP3 mRNA in the cells was observed using RT-real-time PCR. The results demonstrated that trans-caryophyllene and bael leaf oil significantly stimulated keratinocytes to close the wound (p<0.05 vs. vehicle group) and enhanced the level of AQP3 mRNA expression. It suggests that trans-caryophyllene, at least among the components of bael leaf oil, enhances the wound healing action instigated by the bael leaf oil, and that this effect is associated with the water channel aquaporin-3.

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